Identification of protein components of egg masses indicates parental investment in immunoprotection of offspring by Biomphalaria glabrata (Gastropoda, Mollusca)

The macromolecules contributed by the freshwater gastropod Biomphalaria glabrata, intermediate host of Schistosoma mansoni, to developing offspring inside egg masses are poorly known. SDS-PAGE fractionated egg mass fluids (EMF) of M line and BB02 B. glabrata were analyzed by MALDI-TOF (MS and tandem...

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Published in:Developmental and comparative immunology 2010-04, Vol.34 (4), p.425-435
Main Authors: Hathaway, Jennifer J.M., Adema, Coen M., Stout, Barbara A., Mobarak, Charlotte D., Loker, Eric S.
Format: Article
Language:English
Subjects:
Acute-Phase Proteins - immunology
Amino Acid Sequence
Animals
Aplysia
Aplysia - physiology
Bioinformatics
Biomphalaria - parasitology
Biomphalaria - physiology
Biomphalaria glabrata
C-type lectins
Carrier Proteins - immunology
Cu–Zn SOD
Egg Proteins - immunology
Egg Proteins - metabolism
Freshwater mollusc
Gastropoda
Immunity, Innate
Lipopolysaccharide binding protein/bactericidal permeability-increasing protein
Membrane Glycoproteins - immunology
Molecular Sequence Data
Mollusca
Monophenol Monooxygenase - immunology
Pheromone
Pheromones - immunology
Proteomics
Reproduction
Schistosoma mansoni
Schistosoma mansoni - immunology
Schistosomiasis mansoni - immunology
Sequence Homology, Amino Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Superoxide Dismutase - immunology
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Summary:The macromolecules contributed by the freshwater gastropod Biomphalaria glabrata, intermediate host of Schistosoma mansoni, to developing offspring inside egg masses are poorly known. SDS-PAGE fractionated egg mass fluids (EMF) of M line and BB02 B. glabrata were analyzed by MALDI-TOF (MS and tandem MS). A MASCOT database was assembled with EST data from B. glabrata and other molluscs to aid in sequence characterization. Of approximately 20 major EMF polypeptides, 16 were identified as defense-related, including protease inhibitors, a hemocyanin-like factor and tyrosinase (each with possible phenoloxidase activity), extracellular Cu–Zn SOD, two categories of C-type lectins, Gram-negative bacteria-binding protein (GNBP), aplysianin/achacin-like protein, as well as versions of lipopolysaccharide binding protein/bacterial permeability-increasing proteins (LBP/BPI) that differed from those previously described from hemocytes. Along with two sequences that were encoded by “unknown” ESTs, EMF also yielded a compound containing a vWF domain that is likely involved in defense and a polypeptide with homology to the Aplysia pheromone temptin. Further study of B. glabrata pheromones is warranted as these could be useful in efforts to control these schistosome-transmitting snails. Several of the EMF polypeptides were contained in the albumen gland, the organ that produces most EMF. Thus, parental investment of B. glabrata in immunoprotection of its offspring is indicated to be considerable.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2009.12.001