Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers

The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genot...

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Bibliographic Details
Published in:Journal of experimental botany 2010-01, Vol.61 (2), p.453-462
Main Authors: Giorno, Filomena, Wolters-Arts, Mieke, Grillo, Stefania, Scharf, Klaus-Dieter, Vriezen, Wim H, Mariani, Celestina
Format: Article
Language:English
Subjects:
Anther development
Anthers
Biological and medical sciences
Developmental biology
Flowering
Flowers - genetics
Flowers - growth & development
Flowers - physiology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes
heat stress
Heat stress disorders
Heat-Shock Proteins, Small - genetics
Heat-Shock Proteins, Small - metabolism
High temperature
Hot Temperature
HsfA2
Hsp17-CII
Lycopersicon esculentum - genetics
Lycopersicon esculentum - growth & development
Lycopersicon esculentum - physiology
Messenger RNA
Microspores
Plant Proteins - genetics
Plant Proteins - metabolism
Plants
Pollen
RESEARCH PAPER
Research Papers
Stress, Physiological
tomato
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Summary:The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genotype are reported here. A transcriptional analysis at different developmental anther/pollen stages was performed using semi-quantitative and real-time PCR. The messengers were localized using in situ RNA hybridization, and protein accumulation was monitored using immunoblot analysis. Based on the analysis of the gene and protein expression profiles, HsfA2 and Hsp17-CII are finely regulated during anther development and are further induced under both short and prolonged heat stress conditions. These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erp316