Quantification of DNA in Biologic Scaffold Materials

Biological scaffold materials composed of extracellular matrix (ECM) are routinely used for a variety of clinical applications ranging from the treatment of chronic skin ulcers to hernia repair and orthopaedic soft tissue reconstruction. The tissues and species from which the ECM is harvested vary w...

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Bibliographic Details
Published in:The Journal of surgical research 2009-03, Vol.152 (1), p.135-139
Main Authors: Gilbert, Thomas W., Ph.D, Freund, John M., B.S, Badylak, Stephen F., D.V.M., Ph.D., M.D
Format: Article
Language:English
Subjects:
Animals
biologic scaffolds
decellularization
DNA - analysis
ECM
Eosine Yellowish-(YS)
Extracellular Matrix - chemistry
Hematoxylin
Humans
Indoles
Organic Chemicals
Surgery
surgical mesh
Swine
Tissue Scaffolds - chemistry
Tissue Scaffolds - standards
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Summary:Biological scaffold materials composed of extracellular matrix (ECM) are routinely used for a variety of clinical applications ranging from the treatment of chronic skin ulcers to hernia repair and orthopaedic soft tissue reconstruction. The tissues and species from which the ECM is harvested vary widely as do the methods used to remove the cellular component of the source tissues. The efficacy of decellularization procedures can be quantified by examination of the DNA that remains in the ECM. The objective of the present study was to determine the DNA content and fragment length in both laboratory produced and commercially available ECM scaffold materials. Results showed that the majority of DNA is removed from ECM devices but that small amounts remained in most tested materials.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2008.02.013