Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas

AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development sta...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2009-11, Vol.15 (43), p.5425-5431
Main Authors: Xu, Rong, Xia, Biao, Geng, Jie, Shi, Jing, Shi, Hui, Yuan, Li, De, Wei
Format: Article
Language:English
Subjects:
Animals
Exons
Extracellular Matrix - metabolism
Gene Expression Regulation, Developmental
Immunohistochemistry - methods
Insulin-Secreting Cells - cytology
Membrane Proteins - biosynthesis
Membrane Proteins - genetics
Mesoderm - metabolism
Original
Pancreas - embryology
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
SD大鼠
Time Factors
免疫印迹
发展中国家
基因定位
细胞标记
综合征
胰腺癌
逆转录聚合酶链反应
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Summary:AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.15.5425