Evaluation of a Prototype Real-Time PCR Assay for Carcinogenic Human Papillomavirus (HPV) Detection and Simultaneous HPV Genotype 16 (HPV16) and HPV18 Genotyping

Results from a prototype real-time PCR assay that separately detected human papillomavirus genotype 16 (HPV16), HPV18, and 12 other carcinogenic HPV genotypes in aggregate (cobas 4800 HPV test) and results from a PCR assay that detects 37 HPV genotypes individually (Linear Array) were compared using...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-10, Vol.47 (10), p.3344-3347
Main Authors: Castle, Philip E, Sadorra, Mark, Lau, Tiffany, Aldrich, Carrie, Garcia, Francisco A.R, Kornegay, Janet
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cervix Uteri - virology
Female
Fundamental and applied biological sciences. Psychology
Human papillomavirus 16 - classification
Human papillomavirus 16 - genetics
Human papillomavirus 16 - isolation & purification
Human papillomavirus 18 - classification
Human papillomavirus 18 - genetics
Human papillomavirus 18 - isolation & purification
Humans
Medical sciences
Microbiology
Miscellaneous
Papillomaviridae - classification
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomavirus Infections - diagnosis
Papillomavirus Infections - virology
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Tumors
Virology
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Summary:Results from a prototype real-time PCR assay that separately detected human papillomavirus genotype 16 (HPV16), HPV18, and 12 other carcinogenic HPV genotypes in aggregate (cobas 4800 HPV test) and results from a PCR assay that detects 37 HPV genotypes individually (Linear Array) were compared using a convenience sample of cervical specimens (n = 531). The percentage of total agreement between the two assays was 94.7% (95% confidence interval, 92.5 to 96.5%). The Linear Array test was more likely than cobas 4800 HPV test to test positive for the 12 other carcinogenic HPV genotypes among women without evidence of cervical disease (P = 0.004).
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00725-09