Suppression of gastric cancer growth by baculovirus vector-mediated transfer of normal epithelial cell specific-1 gene

To study the inhibitory effect of baculovirus-mediated normal epithelial cell specific-1 (NES1) gene therapy on gastric cancer (GC) in vitro and in vivo. We first constructed recombinant baculovirus vectors and then transfected them into gastric cancer cells (SGC-7901). Efficiency of the baculovirus...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2008-10, Vol.14 (38), p.5810-5815
Main Authors: Huang, Wei, Tian, Xiang-Long, Wu, Yun-Lin, Zhong, Jie, Yu, Li-Fen, Hu, Sheng-Ping, Li, Biao
Format: Article
Language:English
Subjects:
Animals
Baculoviridae - genetics
Cell Line, Tumor
Cell Proliferation
Gastric Cancer
Genes, Reporter
Genetic Therapy - methods
Genetic Vectors
Green Fluorescent Proteins - genetics
Humans
Kallikreins - genetics
Kallikreins - metabolism
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Stomach Neoplasms - therapy
Time Factors
Transduction, Genetic
Transfection
Xenograft Model Antitumor Assays
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Summary:To study the inhibitory effect of baculovirus-mediated normal epithelial cell specific-1 (NES1) gene therapy on gastric cancer (GC) in vitro and in vivo. We first constructed recombinant baculovirus vectors and then transfected them into gastric cancer cells (SGC-7901). Efficiency of the baculovirus for gene transfer into SGC-7901 cells and cell growth curves were detected by fluorescence microscopy, Western blot and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro, respectively. The therapeutic effect of this gene therapy on GC was confirmed in xenografted nude mice. Tumor growth was determined by tumor volume, and expression of NES1 in tumor was analyzed by immunohistochemistry. Baculovirus vectors were successfully transfected into SGC-7901 cells. SGC-7901 cells transfected with the NES1 gene inhibited cell growth. In the Bac-NES1 treated group, tumor growth was significantly reduced with a high level of NES1 expression Baculovirus-mediated NES1 gene can be used in gene therapy for GC.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.14.5810