Genetic modification of stem cells for transplantation

Gene modification of cells prior to their transplantation, especially stem cells, enhances their survival and increases their function in cell therapy. Like the Trojan horse, the gene-modified cell has to gain entrance inside the host's walls and survive and deliver its transgene products Using...

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Bibliographic Details
Published in:Advanced drug delivery reviews 2008-01, Vol.60 (2), p.160-172
Main Authors: Phillips, M. Ian, Tang, Yao Liang
Format: Article
Language:English
Subjects:
Animals
Cancer
Cre/Lox P
Diabetes
Diabetes Mellitus, Type 1 - therapy
Fractures, Bone - therapy
Gene Transfer Techniques
Genetic Therapy - methods
Heart Diseases - therapy
Heart failure
Humans
MicroRNA
Neoplasms - therapy
Neurological diseases
Stem Cell Transplantation - methods
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Vigilant Vector
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Summary:Gene modification of cells prior to their transplantation, especially stem cells, enhances their survival and increases their function in cell therapy. Like the Trojan horse, the gene-modified cell has to gain entrance inside the host's walls and survive and deliver its transgene products Using cellular, molecular and gene manipulation techniques the transplanted cell can be protected in a hostile environment from immune rejection, inflammation, hypoxia and apoptosis. Genetic engineering to modify cells involves constructing modules of functional gene sequences. They can be simple reporter genes or complex cassettes with gene switches, cell specific promoters and multiple transgenes. We discuss methods to deliver and construct gene cassettes with viral and non-viral delivery, siRNA, and conditional Cre/Lox P. We review the current uses of gene-modified stem cells in cardiovascular disease, diabetes, neurological diseases, (including Parkinson's, Alzheimer's and spinal cord injury repair), bone defects, hemophilia, and cancer.
ISSN:0169-409X
1872-8294
DOI:10.1016/j.addr.2007.08.035