DySCo: Quantitating Associations of Membrane Proteins Using Two-Color Single-Molecule Tracking

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capab...

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Bibliographic Details
Published in:Biophysical journal 2009-08, Vol.97 (4), p.L5-L7
Main Authors: Dunne, Paul D., Fernandes, Ricardo A., McColl, James, Yoon, Ji Won, James, John R., Davis, Simon J., Klenerman, David
Format: Article
Language:English
Subjects:
Biophysical Letter
Cells
Cytoskeleton
Equipment Design
Equipment Failure Analysis
Fluorescent Dyes - analysis
Membranes
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Molecular Probe Techniques - instrumentation
Molecules
Protein Interaction Mapping - instrumentation
Protein Interaction Mapping - methods
Proteins
Reproducibility of Results
Sensitivity and Specificity
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Summary:We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2009.05.046