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14-3-3ε protein increases matrix metalloproteinase-2 gene expression via p38 MAPK signaling in NIH3T3 fibroblast cells
One of the 14-3-3 protein isoforms, 14-3-3ε, was previously shown to be increased during skin aging. We suggest here a possible role for the 14-3-3ε protein in skin aging by providing evidence that 14-3-3ε increases the expression of the matrix-metalloproteinase (MMP)-2 gene in NIH3T3 fibroblast cel...
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Published in: | Experimental & molecular medicine 2009-07, Vol.41 (7), p.453-461 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | One of the 14-3-3 protein isoforms, 14-3-3ε, was previously shown to be increased during skin aging. We suggest here a possible role for the 14-3-3ε protein in skin aging by providing evidence that 14-3-3ε increases the expression of the matrix-metalloproteinase (MMP)-2 gene in NIH3T3 fibroblast cells. Expression of the 14-3-3ε gene in NIH3T3 cells primarily up-regulated the expression of the MMP-2 gene at the transcriptional level by inducing specific DNA binding proteins bound to an upstream region of the MMP-2 promoter from -1,629 to -1,612. Inhibition of endogenous 14-3-3ε gene expression by RNA interference also decreased endogenous MMP-2 gene expression. Furthermore, up-regulation of the MMP-2 gene by 14-3-3ε was suppressed by expression of a dominant-negative mutant of p38 MAP kinase. These findings strongly suggest that increased expression of 14-3-3ε contributes to remodeling of extracellular matrix in skin through increasing MMP-2 gene expression via p38 MAP kinase signaling. |
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ISSN: | 1226-3613 |
DOI: | 10.3858/emm.2009.41.7.050 |