A New Methodology to Measure Strength of Adherence of the Fetal Membrane Components, Amnion and the Choriodecidua

Abstract We have previously shown that separation of the amnion from choriodecidua occurs as an integral part of the fetal membranes (FM) rupture process. We have also reported that spontaneous separation of FM is nearly universal with term vaginal delivery. The etiology of this spontaneous FM separ...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2009-06, Vol.30 (6), p.560-563
Main Authors: Kumar, D, Novince, R, Strohl, A, Mercer, B.M, Mansour, J.M, Moore, R.M, Moore, J.J
Format: Article
Language:English
Subjects:
Adhesiveness
Amnion
Amnion - pathology
Amnion - physiology
Biological and medical sciences
Biomechanical Phenomena - physiology
Biomechanical testing equipment
Calibration
Chorion
Chorion - pathology
Chorion - physiology
Decidua - pathology
Decidua - physiology
Diagnostic Techniques, Obstetrical and Gynecological - instrumentation
Embryology: invertebrates and vertebrates. Teratology
Extraembryonic Membranes - pathology
Extraembryonic Membranes - physiology
Female
Fetal membrane adherence
Fetal membranes
Fetal Membranes, Premature Rupture - diagnosis
Fetal Membranes, Premature Rupture - pathology
Fetal Membranes, Premature Rupture - physiopathology
Fundamental and applied biological sciences. Psychology
Humans
Internal Medicine
Obstetrics and Gynecology
Pregnancy
Shear Strength - physiology
T-peel testing
Tensile Strength - physiology
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Summary:Abstract We have previously shown that separation of the amnion from choriodecidua occurs as an integral part of the fetal membranes (FM) rupture process. We have also reported that spontaneous separation of FM is nearly universal with term vaginal delivery. The etiology of this spontaneous FM separation is unknown. If biochemical degradation at the amnion–choriodecidua interface is a factor, decreased adhesive force between the FM components prior to their complete separation would be expected. The purpose of this project was to develop and validate machinery and procedures to measure the adhesive force between amnion and choriodecidua. Commercial tensile testing equipment was adapted to perform a standard T-peel test, per the American Society for Testing and Materials (ASTM) guidelines. FM test strip dimensions, peel speed, and peel force data measurements from force versus displacement curves were optimized for reproducibility. Test system validation was performed using Shurtape CP 60 (slow release painter's masking tape) as the standard. Equipment and procedures for a standard T-peel test on FM were developed. Shurtape CP 60 of decreasing widths showed reproducible, linear changes in the adhesive force range for FM ( r2 = 0.96). The adhesive force between FM components ranged from 0.017 to 0.262 N/cm. Reproducibility was optimal with FM test strips of 4 × 6 cm and a peel speed of 25.4 cm/min. FM showed greater adhesive force adjacent to the placental disc than distal from the disc ( p < 0.05). We have developed equipment and procedures to accurately and reproducibly measure adhesive force between the FM amnion and choriodecidua.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2009.03.014