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Temporal and topographic alterations in expression of the α3 isoform of Na+ ,K+ -ATPase in the rat freeze lesion model of microgyria and epileptogenesis
Abstract Na+ ,K+ -ATPase contributes to the asymmetrical distribution of sodium and potassium ions across the plasma membrane and to maintenance of the membrane potential in many types of cells. Alterations in this protein may play a significant role in many human neurological disorders, including e...
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Published in: | Neuroscience 2009-08, Vol.162 (2), p.339-348 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Abstract Na+ ,K+ -ATPase contributes to the asymmetrical distribution of sodium and potassium ions across the plasma membrane and to maintenance of the membrane potential in many types of cells. Alterations in this protein may play a significant role in many human neurological disorders, including epilepsy. We studied expression of the α3 isoform of Na+ ,K+ -ATPase in the freeze lesion (FL) microgyrus model of developmental epileptogenesis to test the hypothesis that it is downregulated following neonatal cortical injury. FL and sham-operated rat brains were examined at postnatal day (P)7, P10, P14, P21-28 and P50-60 after placement of a transcranial freeze lesion at P0 or P1. Immunohistochemistry and in situ hybridization were used to assess the expression of the α3 isoform of Na+ ,K+ -ATPase (termed α3, or α3 subunit below) in neuropil and the perisomatic areas of pyramidal cells and parvalbumin-containing interneurons. There was a significant decrease ( P |
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ISSN: | 0306-4522 1873-7544 |
DOI: | 10.1016/j.neuroscience.2009.04.003 |