Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

AIM： To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes. METHODS： Cytokine production...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2008-02, Vol.14 (8), p.1192-1203
Main Authors: Kekkonen, Riina-A, Kajasto, Elina, Miettinen, Minja, Veckman, Ville, Korpela, Riitta, Julkunen, Ilkka
Format: Article
Language:English
Subjects:
Anti-Inflammatory Agents - pharmacology
Basic Research
Cytokines - biosynthesis
Cytokines - metabolism
Enzyme-Linked Immunosorbent Assay
Escherichia coli - metabolism
Humans
Interferon-gamma - biosynthesis
Interleukin-12 - biosynthesis
Kinetics
Leuconostoc - metabolism
Leukocytes, Mononuclear - metabolism
Models, Biological
Probiotics - metabolism
Streptococcus thermophilus - metabolism
Time Factors
Tumor Necrosis Factor-alpha - metabolism
乳酸菌
免疫响应
单核细胞
链球菌
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Summary:AIM： To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes. METHODS： Cytokine production in human peripheral blood mononuclear cells （PBMC） in response to stimulation with eleven different potentially probiotic bacterial strains from Streptococcus, Lactobacillus, Bifidobacterium, Lactococcus, L euconostoc a n d Propionibacterium genera was analysed. Production and mRNA expression of TNF-α, IL-12, IFN-y and IL-10 were determined by ELISA and Northern blotting, respectively. RESULTS： All tested bacteria induced TNF-α production. The best inducers of Thl type cytokines IL-12 and IFN-y were Streptococcus and Leuconostoc strains. All BiHdobacterium and Propionibacterium strains induced higher IL-IO production than other studied bacteria. Stimulation of PBMC with any bacterial combinations did not result in enhanced cytokine production suggesting that different bacteria whether gram-positive or gram- negative compete with each other during host cell interactions. CONCLUSION： The probiotic S. thermophilus and Leuconostoc strains are more potent inducers of Thl type cytokines IL-12 and IFN-γ than the probiotic Lactobacillus strains. Bacterial combinations did not result in enhanced cytokine production.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.14.1192