Spectral Analysis of Multiplex Raman Probe Signatures

Raman nanoparticle probes are an emerging new class of optical labels for interrogation of physiological and pathological processes in bioassays, cells, and tissues. Although their unique emission signatures are ideal for multiplexing, the full potential of these probes has not been realized because...

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Bibliographic Details
Published in:ACS nano 2008-11, Vol.2 (11), p.2306-2314
Main Authors: Lutz, Barry R, Dentinger, Claire E, Nguyen, Lienchi N, Sun, Lei, Zhang, Jingwu, Allen, April N, Chan, Selena, Knudsen, Beatrice S
Format: Article
Language:English
Subjects:
Automation
Biological Assay
Fluorescent Dyes - chemistry
Formaldehyde - chemistry
Humans
Keratin-18 - metabolism
Male
Nanoparticles - chemistry
Nanotechnology - methods
Optics and Photonics
Paraffin - chemistry
Prostate-Specific Antigen - metabolism
Prostatic Neoplasms - metabolism
Regression Analysis
Spectrum Analysis, Raman - methods
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Summary:Raman nanoparticle probes are an emerging new class of optical labels for interrogation of physiological and pathological processes in bioassays, cells, and tissues. Although their unique emission signatures are ideal for multiplexing, the full potential of these probes has not been realized because conventional analysis methods are inadequate. We report a novel spectral fitting method that exploits the entire spectral signature to quantitatively extract individual probe signals from multiplex spectra. We evaluate the method in a series of multiplex assays using unconjugated and antibody-conjugated composite organic−inorganic nanoparticles (COINs). Results show sensitive multiplex detection of small signals (
ISSN:1936-0851
1936-086X
DOI:10.1021/nn800243g