Mycobacterium bovis BCG decreases MHC-II expression in vivo on murine lung macrophages and dendritic cells during aerosol infection

Mycobacterium tuberculosis and M. bovis BCG infect APCs. In vitro, mycobacteria inhibit IFN-gamma-induced MHC-II expression by macrophages, but the effects of mycobacteria on lung APCs in vivo remain unclear. To assess MHC-II expression on APCs infected in vivo, mice were aerosol-infected with GFP-e...

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Bibliographic Details
Published in:Cellular immunology 2009-01, Vol.254 (2), p.94-104
Main Authors: Pecora, Nicole D., Fulton, Scott A., Reba, Scott M., Drage, Michael G., Simmons, Daimon P., Urankar-Nagy, Nancy J., Boom, W. Henry, Harding, Clifford V.
Format: Article
Language:English
Subjects:
Animals
Antigen presentation
Antigen-Presenting Cells - immunology
Bone Marrow Cells - cytology
Bone Marrow Cells - immunology
CD11 Antigens - immunology
Cell Differentiation - immunology
Dendritic cell
Dendritic Cells - immunology
Female
Histocompatibility Antigens Class II - immunology
Macrophage
Macrophages, Alveolar - cytology
Macrophages, Alveolar - immunology
MHC
Mice
Mice, Inbred C57BL
Mycobacterium
Mycobacterium bovis
Mycobacterium bovis - immunology
Mycobacterium tuberculosis
Tuberculosis - immunology
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Summary:Mycobacterium tuberculosis and M. bovis BCG infect APCs. In vitro, mycobacteria inhibit IFN-gamma-induced MHC-II expression by macrophages, but the effects of mycobacteria on lung APCs in vivo remain unclear. To assess MHC-II expression on APCs infected in vivo, mice were aerosol-infected with GFP-expressing BCG. At 28 d, ∼1% of lung APCs were GFP+ by flow cytometry and CFU data. Most GFP+ cells were CD11b high/CD11c neg-mid lung macrophages (58–68%) or CD11b high/CD11c high DCs (28–31%). Lung APC MHC-II expression was higher in infected mice than naïve mice. Within infected lungs, however, MHC-II expression was lower in GFP+ cells than GFP− cells for both macrophages and DCs. MHC-II expression was also inhibited on purified lung macrophages and DCs that were infected with BCG in vitro. Thus, lung APCs that harbor mycobacteria in vivo have decreased MHC-II expression relative to uninfected APCs from the same lung, possibly contributing to evasion of T cell responses.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2008.07.002