Biomembrane Sensitivity to Structural Changes in Bound Polymers

Anionic liposomes containing a 4:1 molar ratio of neutral to anionic phospholipids were treated with an excess of five zwitterionic polymers differing only in the spacer length separating their cationic and anionic moieties. Although the polymers do not disrupt the structural integrity of the liposo...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2009-02, Vol.131 (5), p.1666-1667
Main Authors: Yaroslavov, Alexander A, Sitnikova, Tatiana A, Rakhnyanskaya, Anna A, Yaroslavova, Ekaterina G, Davydov, Dmitry A, Burova, Tatiana V, Grinberg, Valery Ya, Shi, Lei, Menger, Fredric M
Format: Article
Language:English
Subjects:
1,2-Dipalmitoylphosphatidylcholine - chemistry
Calorimetry, Differential Scanning
Lipid Bilayers - chemistry
Liposomes - chemistry
Models, Molecular
Phosphatidylcholines - chemistry
Phospholipids - chemistry
Polyvinyls - chemistry
Spectrometry, Fluorescence
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Summary:Anionic liposomes containing a 4:1 molar ratio of neutral to anionic phospholipids were treated with an excess of five zwitterionic polymers differing only in the spacer length separating their cationic and anionic moieties. Although the polymers do not disrupt the structural integrity of the liposomes, they can induce spacer-dependent molecular rearrangements within the liposomes. Thus, the following were observed: spacer length = 1, no binding to the liposomes; spacer length = 2, adsorption to the liposomes, but no molecular rearrangement; spacer length = 3, lateral lipid segregation but little or no flip-flop; spacer length = 4 or 5, lateral lipid segregation and flip-flop. These diverse behaviors are relevant to the use of biomedical formulations where polyelectrolytes play a role.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja808461s