The internal transcribed spacer region of Belonolaimus (Nemata: Belonolaimidae)

Belonalaimus isolates from six U.S. states were compared by restriction endonuclease digestion of amplified first internal transcribed spacer region (ITS1) of the nuclear ribosomal genes. Seven restriction enzymes were selected for evaluation based on restriction sites inferred from the nucleotide s...

Full description

Saved in:
Bibliographic Details
Published in:Journal of nematology 1997-03, Vol.29 (1), p.23-29
Main Authors: Cherry, T. (Lincoln High School, Lincoln, NE.), Szalanski, A.L, Todd, T.C, Powers, T.O
Format: Article
Language:English
Subjects:
ADN
BELONOLAIMUS
CHIMIOTAXONOMIE
NEMATODE DES PLANTES
NEMATODOS DE LAS PLANTAS
QUIMIOTAXONOMIA
RFLP
RIBOSOMAS
RIBOSOME
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Belonalaimus isolates from six U.S. states were compared by restriction endonuclease digestion of amplified first internal transcribed spacer region (ITS1) of the nuclear ribosomal genes. Seven restriction enzymes were selected for evaluation based on restriction sites inferred from the nucleotide sequence of a South Carolina Belonolaimus isolate. Amplified product size from individuals of each isolate was approximately 700 bp. All Midwestern isolates gave distinct restriction digestion patterns. Isolates identified morphologically as Belonolaimus longicaudatus from Florida, South Carolina, and Palm Springs, California, were identical for ITS1 restriction patterns. The correlation between ITS1 restriction patterns and the distribution of B. longicaudatus isolates suggest that the California isolate is a relatively recent introduction into the state
ISSN:0022-300X
2640-396X