TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells

Triggering receptor expressed on myeloid cells-2 (TREM-2) is an innate immune receptor that initiates cellular activation upon ligation. In this study, we examined the interaction of TREM-2 with Neisseria gonorrhoeae using murine TREM-2A, as it has been reported to recognize bacterial ligands. Using...

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Bibliographic Details
Published in:Mucosal immunology 2008-05, Vol.1 (3), p.229-238
Main Authors: Quan, D N, Cooper, M D, Potter, J L, Roberts, M H, Cheng, H, Jarvis, G A
Format: Article
Language:English
Subjects:
Animals
Bacterial Outer Membrane Proteins - immunology
Cell Line
Epithelial Cells - immunology
Epithelial Cells - metabolism
Epithelial Cells - microbiology
Fallopian Tubes - immunology
Fallopian Tubes - metabolism
Fallopian Tubes - microbiology
Female
Humans
Interleukin-6 - biosynthesis
Lipopolysaccharides - immunology
Mice
Neisseria gonorrhoeae - immunology
Protein Binding - immunology
Receptors, Immunologic - biosynthesis
Receptors, Immunologic - immunology
Signal Transduction
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Summary:Triggering receptor expressed on myeloid cells-2 (TREM-2) is an innate immune receptor that initiates cellular activation upon ligation. In this study, we examined the interaction of TREM-2 with Neisseria gonorrhoeae using murine TREM-2A, as it has been reported to recognize bacterial ligands. Using a whole-bacteria enzyme-linked immunosorbent assay (ELISA), TREM-2A bound to all six strains in variable degrees. Far-western blots of gonococcal outer membranes revealed TREM-2A binding to lipooligosaccharide (LOS) and opacity (Opa) protein, with predominant binding to LOS. Binding of TREM-2A to LOS was confirmed by ELISA and surface plasmon resonance. O-deacylation of the lipid A significantly reduced binding. Flow cytometry and reporter cell assays showed that gonococci bound to TREM-2A-transfected cells and induced transmembrane signaling. In humans, TREM-2 was constitutively expressed by genitourinary and fallopian tube epithelial cells, both of which are primary targets of gonococcal invasion. Ligation of TREM-2 by LOS induced interleukin-6 production in HeLa cervical carcinoma cells. To our knowledge, this is the first report of the expression of human TREM-2 by cells deriving from a non-myeloid lineage. We conclude that gonococci can interact with TREM-2 receptors through binding to LOS and Opa protein and initiate cell signaling and cytokine production.
ISSN:1933-0219
1935-3456
DOI:10.1038/mi.2008.1