The 5' external transcribed spacer in mouse ribosomal RNA contains two cleavage sites

The 5' external transcribed spacer (5'ETS) is critical for 18S rRNA formation and is the longest noncoding region in a ribosomal RNA transcript. Here we show that processing in mouse 5'ETS involves two cleavage events. Processing at site A' corresponds to the previously described...

Full description

Saved in:
Bibliographic Details
Published in:RNA (Cambridge) 2009-01, Vol.15 (1), p.14-20
Main Authors: Kent, Tatyana, Lapik, Yevgeniya R, Pestov, Dimitri G
Format: Article
Language:English
Subjects:
Animals
DNA, Ribosomal Spacer - chemistry
Exoribonucleases - chemistry
Exoribonucleases - metabolism
Exosome Multienzyme Ribonuclease Complex
Exosomes - metabolism
Humans
Mice
Models, Biological
Models, Genetic
Molecular Sequence Data
NIH 3T3 Cells
RNA Precursors - chemistry
RNA Precursors - metabolism
RNA Splicing - physiology
RNA, Ribosomal - chemistry
RNA, Ribosomal - metabolism
RNA, Ribosomal, 18S - chemistry
RNA, Ribosomal, 18S - metabolism
RNA, Small Nucleolar - metabolism
RNA, Untranslated - chemistry
RNA, Untranslated - metabolism
Saccharomyces cerevisiae Proteins - chemistry
Saccharomyces cerevisiae Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The 5' external transcribed spacer (5'ETS) is critical for 18S rRNA formation and is the longest noncoding region in a ribosomal RNA transcript. Here we show that processing in mouse 5'ETS involves two cleavage events. Processing at site A' corresponds to the previously described "primary cleavage," which precedes other processing steps. Processing at the novel site A0 occurs 1 kb downstream from A' yielding two new rRNA precursors: 43S and 29S. The excised 5'-A' and A'-A0 fragments are rapidly degraded under normal conditions. Depletion of the exosome component EXOSC10/PM-Scl100 (ortholog of yeast Rrp6p) results in a strong accumulation of the A'-A0 spacer fragment in mouse cells. We discuss the finding of a second processing site in mammalian 5'ETS in relation to the involvement of the U3 snoRNA in pre-rRNA processing and present a revised map of the mouse 18S rRNA processing pathway.
ISSN:1355-8382
1469-9001
DOI:10.1261/rna.1384709