Collecting Duct Renin Is Upregulated in Both Kidneys of 2-Kidney, 1-Clip Goldblatt Hypertensive Rats

Renin in collecting duct cells is upregulated in chronic angiotensin II–infused rats via angiotensin II type 1 receptors. To determine whether stimulation of collecting duct renin is a blood pressure–dependent effect; changes in collecting duct renin and associated parameters were assessed in both k...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 2008-06, Vol.51 (6), p.1590-1596
Main Authors: Prieto-Carrasquero, Minolfa C, Botros, Fady T, Pagan, Javier, Kobori, Hiroyuki, Seth, Dale M, Casarini, Dulce E, Navar, L Gabriel
Format: Article
Language:English
Subjects:
Angiotensin I - metabolism
Angiotensin II - blood
Angiotensin II - pharmacology
Animals
Arterial hypertension. Arterial hypotension
Biological and medical sciences
Blood and lymphatic vessels
Blood Pressure
Body Weight
Cardiology. Vascular system
Disease Models, Animal
Experimental diseases
Hypertension, Renovascular - drug therapy
Hypertension, Renovascular - metabolism
Immunohistochemistry
Juxtaglomerular Apparatus - metabolism
Kidney Cortex - metabolism
Kidney Medulla - metabolism
Kidney Tubules, Collecting - metabolism
Male
Medical sciences
Rats
Rats, Sprague-Dawley
Renal Artery
Renin - blood
Renin - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Surgical Instruments
Up-Regulation - physiology
Vasoconstrictor Agents - blood
Vasoconstrictor Agents - pharmacology
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Summary:Renin in collecting duct cells is upregulated in chronic angiotensin II–infused rats via angiotensin II type 1 receptors. To determine whether stimulation of collecting duct renin is a blood pressure–dependent effect; changes in collecting duct renin and associated parameters were assessed in both kidneys of 2-kidney, 1-clip Goldblatt hypertensive (2K1C) rats. Renal medullary tissues were used to avoid the contribution of renin from juxtaglomerular cells. Systolic blood pressure increased to 184±9 mm Hg in 2K1C rats (n=19) compared with sham rats (121±6 mm Hg; n=12). Although renin immunoreactivity markedly decreased in juxtaglomerular cells of nonclipped kidneys (NCK0.2±0.0 versus 1.0±0.0 relative ratio) and was augmented in clipped kidneys (CK1.7±1.0 versus 1.0±0.0 relative ratio), its immunoreactivity increased in cortical and medullary collecting ducts of both kidneys of 2K1C rats (CK2.8±1.0 cortex; 2.1±1.0 medulla; NCK4.6±2.0 cortex, 3.2±1.0 medulla versus 1.0±0.0 in sham kidneys). Renal medullary tissues of 2K1C rats showed greater levels of renin protein (CK1.4±0.2; NCK1.5±0.3), renin mRNA (CK5.8±2.0; NCK4.9±2.0), angiotensin I (CK120±18 pg/g; NCK129±13 pg/g versus sham67±6 pg/g), angiotensin II (CK150±32 pg/g; NCK123±21 pg/g versus sham91±12 pg/g; P
ISSN:0194-911X
1524-4563
DOI:10.1161/HYPERTENSIONAHA.108.110916