Protein dynamics from time resolved UV Raman spectroscopy

Raman spectroscopy can provide unique information on the evolution of structure in proteins over a wide range of time scales; the picosecond to millisecond range can be accessed with pump–probe techniques. Specific parts of the molecule are interrogated by tuning the probe laser to a resonant electr...

Full description

Saved in:
Bibliographic Details
Published in:Current opinion in structural biology 2008-10, Vol.18 (5), p.623-629
Main Authors: Balakrishnan, Gurusamy, Weeks, Colin L, Ibrahim, Mohammed, Soldatova, Alexandra V, Spiro, Thomas G
Format: Article
Language:English
Subjects:
Models, Molecular
Protein Conformation
Protein Denaturation
Protein Folding
Proteins - chemistry
Scattering, Radiation
Sensitivity and Specificity
Spectrum Analysis, Raman - methods
Thermodynamics
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Raman spectroscopy can provide unique information on the evolution of structure in proteins over a wide range of time scales; the picosecond to millisecond range can be accessed with pump–probe techniques. Specific parts of the molecule are interrogated by tuning the probe laser to a resonant electronic transition, including the UV transitions of aromatic residues and of the peptide bond. Advances in laser technology have enabled the characterization of transient species at an unprecedented level of structural detail. Applications to protein unfolding and allostery are reviewed.
ISSN:0959-440X
1879-033X
DOI:10.1016/j.sbi.2008.06.001