In Situ Enrichment of Phosphopeptides on MALDI Plates Functionalized by Reactive Landing of Zirconium(IV)−n-Propoxide Ions

We report substantial in situ enrichment of phosphopeptides in peptide mixtures using zirconium oxide coated plates for detection by MALDI-TOF mass spectrometry. The novel feature of this approach rests on the specific preparation of zirconium oxide coatings using reactive landing on stainless steel...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2007-07, Vol.79 (14), p.5449-5456
Main Authors: Blacken, Grady R, Volný, Michael, Vaisar, Tomáš, Sadílek, Martin, Tureček, František
Format: Article
Language:English
Subjects:
1-Propanol - chemistry
Amino Acid Sequence
Analytical chemistry
Caseins - chemistry
Chemistry
Exact sciences and technology
Ions - chemistry
Mass spectrometry
Molecular Sequence Data
Peptides
Phosphopeptides - analysis
Solutions - chemistry
Spectrometric and optical methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Stainless Steel - chemistry
Surface Properties
Trypsin - chemistry
Zirconium
Zirconium - chemistry
Zirconium oxides
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Summary:We report substantial in situ enrichment of phosphopeptides in peptide mixtures using zirconium oxide coated plates for detection by MALDI-TOF mass spectrometry. The novel feature of this approach rests on the specific preparation of zirconium oxide coatings using reactive landing on stainless steel support of gas-phase positive ions produced by electrospray of zirconium(IV)−n-propoxide solutions in 1-propanol. Reactive landing was found to produce durable functionalized surfaces for selective phosphopeptide capture and desorption−ionization by MALDI. Enrichment factors on the order of 20−90 were achieved for several monophosphorylated peptides relative to abundant nonphosphorylated peptides in tryptic digests. We demonstrate the ability of the zirconium oxide functionalized MALDI surfaces to facilitate detection of enriched phosphopeptides in mid-femtomole amounts of α-casein digests per MALDI spot.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac070790w