Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation

Production of a red blood cell's hemoglobin depends on mitochondrial heme synthesis. However, mature red blood cells are devoid of mitochondria and rely on glycolysis for ATP production. The molecular basis for the selective elimination of mitochondria from mature red blood cells remains contro...

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Bibliographic Details
Published in:Blood 2008-08, Vol.112 (4), p.1493-1502
Main Authors: Kundu, Mondira, Lindsten, Tullia, Yang, Chia-Ying, Wu, Junmin, Zhao, Fangping, Zhang, Ji, Selak, Mary A., Ney, Paul A., Thompson, Craig B.
Format: Article
Language:English
Subjects:
Animals
Autophagy
Autophagy-Related Protein-1 Homolog
Cell Differentiation
Erythrocytes - cytology
Mice
Mitochondria - metabolism
Protein-Serine-Threonine Kinases - physiology
Red Cells
Reticulocytes - cytology
Ribosomes - metabolism
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Summary:Production of a red blood cell's hemoglobin depends on mitochondrial heme synthesis. However, mature red blood cells are devoid of mitochondria and rely on glycolysis for ATP production. The molecular basis for the selective elimination of mitochondria from mature red blood cells remains controversial. Recent evidence suggests that clearance of both mitochondria and ribosomes, which occurs in reticulocytes following nuclear extrusion, depends on autophagy. Here, we demonstrate that Ulk1, a serine threonine kinase with homology to yeast atg1p, is a critical regulator of mitochondrial and ribosomal clearance during the final stages of erythroid maturation. However, in contrast to the core autophagy genes such as atg5 and atg7, expression of ulk1 is not essential for induction of macroautophagy in response to nutrient deprivation or for survival of newborn mice. Together, these data suggest that the ATG1 homologue, Ulk1, is a component of the selective autophagy machinery that leads to the elimination of organelles in erythroid cells rather that an essential mechanistic component of autophagy.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2008-02-137398