Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles

Cultured lung cells provide an alternative to animal exposures for comparing the effects of different types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory cytokine signaling in response to many types of environmental particles, but there have been fe...

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Bibliographic Details
Published in:Toxicology in vitro 2008-03, Vol.22 (2), p.498-509
Main Authors: Veranth, John M., Cutler, N. Shane, Kaser, Erin G., Reilly, Christopher A., Yost, Garold S.
Format: Article
Language:English
Subjects:
A549
Animals
BEAS-2B
Cell Survival - drug effects
Cells, Cultured
Coal
Culture Media
Cytokines - biosynthesis
Dust
Environmental dust
Environmental Pollutants - chemistry
Environmental Pollutants - toxicity
Enzyme-Linked Immunosorbent Assay
Epithelial cell
Human
Humans
Interleukin-6
Interleukin-6 - metabolism
Lung
Macrophage
Mice
Particulate matter
Particulate Matter - chemistry
Particulate Matter - toxicity
Rats
RAW264.7
Soil
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Summary:Cultured lung cells provide an alternative to animal exposures for comparing the effects of different types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory cytokine signaling in response to many types of environmental particles, but there have been few studies comparing identical treatments in multiple cell types or identical cells with alternative cell culture protocols. We compared soil-derived, diesel, coal fly ash, titanium dioxide, and kaolin particles along with soluble vanadium and lipopolysaccharide, applied to airway-derived cells grown in submerged culture. Cell types included A549, BEAS-2B, RAW 264.7, and primary macrophages. The cell culture models (specific combinations of cell types and culture conditions) were reproducibly different in the cytokine signaling responses to the suite of treatments. Further, Interleukin-6 (IL-6) response to the treatments changed when the same cells, BEAS-2B, were grown in KGM versus LHC-9 media or in media containing bovine serum. The effect of changing media composition was reversible over multiple changes of media type. Other variables tested included culture well size and degree of confluence. The observation that sensitivity of a cell type to environmental agonists can be manipulated by modifying culture conditions suggests a novel approach for studying biochemical mechanisms of particle toxicity.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2007.10.011