The expression of MDM2/CDK4 gene product in the differential diagnosis of well differentiated liposarcoma and large deep-seated lipoma

Ordinary lipomas are cytogenetically characterized by a variety of balanced rearrangements involving chromosome segment 12q13-15, whereas well differentiated liposarcomas (WDL) show supernumerary ring and giant marker chromosomes, known to contain amplified 12q sequences. The tight correlation betwe...

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Bibliographic Details
Published in:British journal of cancer 2000-04, Vol.82 (7), p.1271-1275
Main Authors: PILOTTI, S, DELLA TORRE, G, MEZZELANI, A, TAMBORINI, E, AZZARELLI, A, SOZZI, G, PIEROTTI, M
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Blotting, Southern
Cancer research
CDK4 gene
Chemotherapy
Chromosomes
Cyclin-Dependent Kinases - genetics
Dermatology
Diagnosis, Differential
Female
Gene Amplification
Genes
Humans
Immunohistochemistry
Immunophenotyping
In Situ Hybridization, Fluorescence
lipoma
Lipoma - diagnosis
Lipoma - genetics
Liposarcoma
Liposarcoma - diagnosis
Liposarcoma - genetics
Male
MDM2 gene
Medical research
Medical sciences
Middle Aged
Radiation therapy
Regular
Tumors
Tumors of the skin and soft tissue. Premalignant lesions
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Summary:Ordinary lipomas are cytogenetically characterized by a variety of balanced rearrangements involving chromosome segment 12q13-15, whereas well differentiated liposarcomas (WDL) show supernumerary ring and giant marker chromosomes, known to contain amplified 12q sequences. The tight correlation between the presence of ring chromosomes and both amplification and overexpression of MDM2 and CDK4 genes suggests the exploration of the possibility that immunocytochemistry (ICC) might assist in the differential diagnosis of lipoma-like well differentiated liposarcomas (LL-WDL) and large deep-seated lipomas (LDSL). For this purpose, 21 cases of the former and 19 cases of the latter tumours were analysed by ICC and, according to the availability of material, by molecular and cytogenetic approaches. All lipomas displayed a null MDM2/CDK4 phenotype, whereas all LL-WDL showed MDM2/CDK4 or CDK4 phenotypes. Southern blot analysis performed on 16 suitable cases, complemented by fluorescence in situ hybridization and classical cytogenetic analysis in 11 cases, was consistent with, and further supported the immunophenotyping data. In conclusion, MDM2/CDK4 product-based immunophenotyping appears to represent a valuable method for the categorization of arguable LDSL.
ISSN:0007-0920
1532-1827
DOI:10.1054/bjoc.1999.1090