The aptamer core of SAM-IV riboswitches mimics the ligand-binding site of SAM-I riboswitches

A novel family of riboswitches, called SAM-IV, is the fourth distinct set of mRNA elements to be reported that regulate gene expression via direct sensing of S-adenosylmethionine (SAM or AdoMet). SAM-IV riboswitches share conserved nucleotide positions with the previously described SAM-I riboswitche...

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Bibliographic Details
Published in:RNA (Cambridge) 2008-05, Vol.14 (5), p.822-828
Main Authors: Weinberg, Zasha, Regulski, Elizabeth E, Hammond, Ming C, Barrick, Jeffrey E, Yao, Zizhen, Ruzzo, Walter L, Breaker, Ronald R
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - genetics
Aptamers, Nucleotide - metabolism
Base Sequence
Binding Sites - genetics
DNA Primers - genetics
Evolution, Molecular
Ligands
Nucleic Acid Conformation
RNA, Bacterial - chemistry
RNA, Bacterial - genetics
RNA, Bacterial - metabolism
RNA, Messenger - chemistry
RNA, Messenger - classification
RNA, Messenger - genetics
RNA, Messenger - metabolism
S-Adenosylmethionine - metabolism
Streptomyces coelicolor - genetics
Streptomyces coelicolor - metabolism
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Summary:A novel family of riboswitches, called SAM-IV, is the fourth distinct set of mRNA elements to be reported that regulate gene expression via direct sensing of S-adenosylmethionine (SAM or AdoMet). SAM-IV riboswitches share conserved nucleotide positions with the previously described SAM-I riboswitches, despite rearranged structures and nucleotide positions with family-specific nucleotide identities. Sequence analysis and molecular recognition experiments suggest that SAM-I and SAM-IV riboswitches share similar ligand binding sites, but have different scaffolds. Our findings support the view that RNA has considerable structural versatility and reveal that riboswitches exploit this potential to expand the scope of RNA in genetic regulation.
ISSN:1355-8382
1469-9001
DOI:10.1261/rna.988608