Novel method for rapid identification of Nocardia species by detection of preformed enzymes

The purpose of the present study was to devise a method for the identification of Nocardia species that is more technically simple, accurate, and rapid than current standard methods of identification. We focused on a commercial bacteria identification system that contained chromogenic test substrate...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1996, Vol.34 (1), p.103-107
Main Authors: Biehle, J.R, Cavalieri, S.J, Felland, T, Zimmer, B.L
Format: Article
Language:English
Subjects:
animal diseases
animal health
bacterial infections
Bacteriological methods and techniques used in bacteriology
Bacteriological Techniques
Bacteriology
Biological and medical sciences
Chromogenic Compounds
Evaluation Studies as Topic
Fundamental and applied biological sciences. Psychology
human health and safety
Humans
medicine
Microbiology
Nocardia
Nocardia - classification
Nocardia - enzymology
Nocardia - isolation & purification
Nocardia asteroides - enzymology
Nocardia asteroides - isolation & purification
Nocardia brasiliensis
Nocardia farcinica
Nocardia nova
Reproducibility of Results
Species Specificity
Substrate Specificity
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Summary:The purpose of the present study was to devise a method for the identification of Nocardia species that is more technically simple, accurate, and rapid than current standard methods of identification. We focused on a commercial bacteria identification system that contained chromogenic test substrates. Two MicroScan products were selected for use in the study on the basis of their content of chromogenic and conventional substrates. They were the Rapid Anaerobe Identification and the HNID panels. A total of 85 strains of Nocardia representing five species were used in the study. All isolates were identified as Nocardia species by the use of standard methods. The beta-naphthylamide-labeled substrate L-pyrrolidonyl-beta-naphthylamide (PYR), the nitrophenyl-labeled substrate p-nitrophenyl-alpha-D-mannopyranoside (MNP), and indoxyl phosphate were found to be useful for identification purposes. N. farcinica and N. nova were the only species positive for PYR, whereas N. brasiliensis was the only species that hydrolyzed MNP. All strains of N. brasiliensis, N. otitidiscavarium, and N. farcinica were positive for indoxyl phosphate, whereas strains of N. nova and N. asteroides sensu stricto were always negative. Agreement between the standard and enzymatic identification methods was 100%. In summary, detection of preformed enzymes appears to be a simple and reproducible method for the identification of Nocardia spp.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.34.1.103-107.1996