Carbohydrate ingestion attenuates the increase in plasma interleukin-6, but not skeletal muscle interleukin-6 mRNA, during exercise in humans

The present study was undertaken to examine the effects of exercise and carbohydrate (CHO) ingestion on interleukin-6 (IL-6) gene expression in skeletal muscle and plasma IL-6 concentration. Seven moderately trained men completed 60 min of exercise at a workload corresponding to each individual'...

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Bibliographic Details
Published in:The Journal of physiology 2001-06, Vol.533 (2), p.585-591
Main Authors: Starkie, R. L., Arkinstall, M. J., Koukoulas, I., Hawley, J. A., Febbraio, M. A.
Format: Article
Language:English
Subjects:
Adult
Biopsy
Blood Glucose
Dietary Carbohydrates - administration & dosage
Exercise - physiology
Exercise Test
Gene Expression - physiology
Glycogen - analysis
Humans
Interleukin-6 - blood
Interleukin-6 - genetics
Male
Muscle, Skeletal - chemistry
Muscle, Skeletal - cytology
Muscle, Skeletal - physiology
Original
RNA, Messenger - analysis
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Summary:The present study was undertaken to examine the effects of exercise and carbohydrate (CHO) ingestion on interleukin-6 (IL-6) gene expression in skeletal muscle and plasma IL-6 concentration. Seven moderately trained men completed 60 min of exercise at a workload corresponding to each individual's lactate threshold on four randomised occasions. Two trials were conducted on a bicycle ergometer (Cyc) and two on a running treadmill (Run) either with (CHO) or without (Con) the ingestion of a CHO beverage throughout the exercise. Muscle biopsies were obtained from the vastus lateralis before and immediately after exercise and IL-6 gene expression in these samples was determined using real-time PCR. In addition, venous blood samples were collected at rest, and after 30 min during and at the cessation of exercise. These samples were analysed for plasma IL-6. Irrespective of exercise mode or CHO ingestion, exercise resulted in a 21 ± 4-fold increase ( P < 0.01; main exercise effect) in IL-6 mRNA expression. In contrast, while the mode of exercise did not affect the exercise-induced increase in plasma IL-6, CHO ingestion blunted ( P < 0.01) this response. These data demonstrate that CHO ingestion attenuates the plasma IL-6 concentration during both cycling and running exercise. However, because IL-6 mRNA expression was unaffected by CHO ingestion, it is likely that the ingestion of CHO during exercise attenuates IL-6 production by tissues other than skeletal muscle.
ISSN:0022-3751
1469-7793
DOI:10.1111/j.1469-7793.2001.0585a.x