Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6

Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6

Plasma interleukin (IL)-6 concentration is increased with exercise and it has been demonstrated that contracting muscles can produce IL- The question addressed in the present study was whether the IL-6 production by contracting skeletal muscle is of such a magnitude that it can account for the IL-6...

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Bibliographic Details
Published in:The Journal of physiology 2000-11, Vol.529 (1), p.237-242
Main Authors: Steensberg, Adam, Hall, Gerrit, Osada, Takuya, Sacchetti, Massimo, Saltin, Bengt, Pedersen, Bente Klarlund
Format: Article
Language:eng
Subjects:
Adult
Ergometry
Exercise - physiology
Humans
Interleukin-6 - biosynthesis
Interleukin-6 - blood
Leg - blood supply
Male
Muscle Contraction - physiology
Muscle, Skeletal - metabolism
Rapid Report
Regional Blood Flow - physiology
Time Factors
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Summary:Plasma interleukin (IL)-6 concentration is increased with exercise and it has been demonstrated that contracting muscles can produce IL- The question addressed in the present study was whether the IL-6 production by contracting skeletal muscle is of such a magnitude that it can account for the IL-6 accumulating in the blood. This was studied in six healthy males, who performed one-legged dynamic knee extensor exercise for 5 h at 25 W, which represented 40% of peak power output ( W max ). Arterial-femoral venous (a-fv) differences over the exercising and the resting leg were obtained before and every hour during the exercise. Leg blood flow was measured in parallel by the ultrasound Doppler technique. IL-6 was measured by enzyme-linked immunosorbent assay (ELISA). Arterial plasma concentrations for IL-6 increased 19-fold compared to rest. The a-fv difference for IL-6 over the exercising leg followed the same pattern as did the net IL-6 release. Over the resting leg, there was no significant a-fv difference or net IL-6 release. The work was produced by 2.5 kg of active muscle, which means that during the last 2 h of exercise, the median IL-6 production was 6.8 ng min −1 (kg active muscle) −1 (range, 3.96-9.69 ng min −1 kg −1 ). The net IL-6 release from the muscle over the last 2 h of exercise was 17-fold higher than the elevation in arterial IL-6 concentration and at 5 h of exercise the net release during 1 min was half of the IL-6 content in the plasma. This indicates a very high turnover of IL-6 during muscular exercise. We suggest that IL-6 produced by skeletal contracting muscle contributes to the maintenance of glucose homeostasis during prolonged exercise.
ISSN:0022-3751
1469-7793