Neurite arborization and mosaic spacing in the mouse retina require DSCAM

To establish functional circuitry, retinal neurons occupy spatial domains by arborizing their processes, which requires the self-avoidance of neurites from an individual cell, and by spacing their cell bodies, which requires positioning the soma and establishing a zone within which other cells of th...

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Bibliographic Details
Published in:Nature 2008-01, Vol.451 (7177), p.470-474
Main Authors: Burgess, Robert W, Fuerst, Peter G, Koizumi, Amane, Masland, Richard H
Format: Article
Language:English
Subjects:
Abnormalities
Amacrine Cells - cytology
Amacrine Cells - metabolism
Animals
Biological and medical sciences
Cell adhesion & migration
Cell Adhesion Molecules
Cell Movement
Cellular biology
Chemical properties
Chromosome aberrations
Down syndrome
Drosophila
Eye and associated structures. Visual pathways and centers. Vision
Fundamental and applied biological sciences. Psychology
Gene Deletion
Medical genetics
Medical sciences
Mice
Mutation
Neurites - physiology
Physiological aspects
Proteins - genetics
Proteins - metabolism
Retina
Retina - cytology
Retina - embryology
Retina - metabolism
Retina - pathology
RNA Stability
Rodents
Structure
Vertebrates: nervous system and sense organs
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Summary:To establish functional circuitry, retinal neurons occupy spatial domains by arborizing their processes, which requires the self-avoidance of neurites from an individual cell, and by spacing their cell bodies, which requires positioning the soma and establishing a zone within which other cells of the same type are excluded. The mosaic patterns of distinct cell types form independently and overlap. The cues that direct these processes in the vertebrate retina are not known. Here we show that some types of retinal amacrine cells from mice with a spontaneous mutation in Down syndrome cell adhesion molecule (Dscam), a gene encoding an immunoglobulin-superfamily member adhesion molecule, have defects in the arborization of processes and in the spacing of cell bodies. In the mutant retina, cells that would normally express Dscam have hyperfasciculated processes, preventing them from creating an orderly arbor. Also, their cell bodies are randomly distributed or pulled into clumps rather than being regularly spaced mosaics. Our results indicate that mouse DSCAM mediates isoneuronal self-avoidance for arborization and heteroneuronal self-avoidance within specific cell types to prevent fasciculation and to preserve mosaic spacing. These functions are analogous to those of Drosophila DSCAM (ref. 6) and DSCAM2 (ref. 7). DSCAM may function similarly in other regions of the mammalian nervous system, and this role may extend to other members of the mammalian Dscam gene family.
ISSN:0028-0836
1476-4687
1476-4679
DOI:10.1038/nature06514