Determination of the most closely related bacillus isolates to Bacillus anthracis by multilocus sequence typing

There have been many efforts to develop Bacillus anthracis detection assays, but the problem of false-positive results has often been encountered. Therefore, to validate an assay for B. anthracis detection, it is critical to examine its specificity with the most closely related Bacillus isolates tha...

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Bibliographic Details
Published in:The Yale journal of biology & medicine 2005-01, Vol.78 (1), p.1-14
Main Authors: Kim, Kijeong, Cheon, Eunhee, Wheeler, Katherine E, Youn, Youngchul, Leighton, Terrance J, Park, Chulmin, Kim, Wonyong, Chung, Sang-In
Format: Article
Language:English
Subjects:
Bacillus
Bacillus anthracis
Bacillus anthracis - classification
Bacillus anthracis - genetics
Bacillus anthracis - isolation & purification
Bacterial Typing Techniques - methods
Chromosome Mapping - methods
DNA Mutational Analysis - methods
Genetic Variation - genetics
Linkage Disequilibrium - genetics
Multilocus sequence typing
Phylogeny
Polymorphism, Single Nucleotide - genetics
Sequence Analysis, DNA - methods
Species Specificity
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Summary:There have been many efforts to develop Bacillus anthracis detection assays, but the problem of false-positive results has often been encountered. Therefore, to validate an assay for B. anthracis detection, it is critical to examine its specificity with the most closely related Bacillus isolates that are available. To define the most closely related Bacillus isolates to B. anthracis in our Bacillus collections, we analyzed by multilocus sequence typing (MLST) the phylogeny of 77 closely related Bacillus isolates selected from 264 Bacillus isolates. The selection includes all the Bacillus isolates that have been shown in our previous studies to produce false-positive results by some anthrax-detection assays. The MLST phylogenetic analyses revealed that 27 of the non-B. anthracis isolates clustered within the B. anthracis clade, and four of them (three sequence types, STs) had the highest degree of genetic relatedness with B. anthracis, 18 (11 STs) had the second highest, and five (five STs) had the third highest. We anticipate that the inclusion of the 19 ST isolates when analyzing B. anthracis detection assays will prove to be useful for screening for their specificity to detect B. anthracis.
ISSN:0044-0086
1551-4056