Crystallization and preliminary crystallographic analysis of orotidine 5′-monophosphate decarboxylase from the human malaria parasite Plasmodium falciparum

Orotidine 5′‐monophosphate (OMP) decarboxylase (OMPDC; EC 4.1.1.23) catalyzes the final step in the de novo synthesis of uridine 5′‐monophosphate (UMP) and defects in the enzyme are lethal in the malaria parasite Plasmodium falciparum. Active recombinant P. falciparum OMPDC (PfOMPDC) was crystallize...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2006-06, Vol.62 (6), p.542-545
Main Authors: Krungkrai, Sudaratana R., Tokuoka, Keiji, Kusakari, Yukiko, Inoue, Tsuyoshi, Adachi, Hiroaki, Matsumura, Hiroyoshi, Takano, Kazufumi, Murakami, Satoshi, Mori, Yusuke, Kai, Yasushi, Krungkrai, Jerapan, Horii, Toshihiro
Format: Article
Language:English
Subjects:
Animals
CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
Crystallization Communications
CRYSTALS
DEFECTS
DIFFRACTION
DIMERS
orotidine 5′-monophosphate decarboxylase
Orotidine-5'-Phosphate Decarboxylase - chemistry
Plasmodium falciparum
Plasmodium falciparum - enzymology
Recombinant Proteins
RESOLUTION
SOLVENTS
SPACE GROUPS
SYMMETRY
SYNCHROTRON RADIATION
SYNTHESIS
X-Ray Diffraction
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Description
Summary:Orotidine 5′‐monophosphate (OMP) decarboxylase (OMPDC; EC 4.1.1.23) catalyzes the final step in the de novo synthesis of uridine 5′‐monophosphate (UMP) and defects in the enzyme are lethal in the malaria parasite Plasmodium falciparum. Active recombinant P. falciparum OMPDC (PfOMPDC) was crystallized by the seeding method in a hanging drop using PEG 3000 as a precipitant. A complete set of diffraction data from a native crystal was collected to 2.7 Å resolution at 100 K using synchrotron radiation at the Swiss Light Source. The crystal exhibits trigonal symmetry (space group R3), with hexagonal unit‐cell parameters a = b = 201.81, c = 44.03 Å. With a dimer in the asymmetric unit, the solvent content is 46% (VM = 2.3 Å3 Da−1).
ISSN:1744-3091
1744-3091
DOI:10.1107/S1744309106015594