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Control of pulsatile 5-HT/insulin secretion from single mouse pancreatic islets by intracellular calcium dynamics
Glucose-induced insulin release from single islets of Langerhans is pulsatile. We have investigated the correlation between changes in cytosolic free calcium concentration ([Ca 2+ ] i ) and oscillatory insulin secretion from single mouse islets, in particular examining the basis for differences in s...
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Published in: | The Journal of physiology 1998-07, Vol.510 (1), p.135-143 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Glucose-induced insulin release from single islets of Langerhans is pulsatile. We have investigated the correlation between
changes in cytosolic free calcium concentration ([Ca 2+ ] i ) and oscillatory insulin secretion from single mouse islets, in particular examining the basis for differences in secretory
responses to intermediate and high glucose concentrations. Insulin release was monitored in real time through the amperometric
detection of the surrogate insulin marker 5-hydroxytryptamine (5-HT) via carbon fibre microelectrodes. The [Ca 2+ ] i was simultaneously recorded by whole-islet fura-2 microfluorometry.
In 82 % of the experiments, exposure to 11 mM glucose evoked regular high-frequency (average, 3.4 min â1 ) synchronous oscillations in amperometric current and [Ca 2+ ] i . In the remaining experiments (18 %), 11 mM glucose induced an oscillatory pattern consisting of high-frequency [Ca 2+ ] i oscillations that were superimposed on low-frequency (average, 0.32 min â1 ) [Ca 2+ ] i waves. Intermittent high-frequency [Ca 2+ ] i oscillations gave rise to a similar pattern of pulsatile 5-HT release.
Raising the glucose concentration from 11 to 20 mM increased the duration of the steady-state [Ca 2+ ] i oscillations without increasing their amplitude. In contrast, both the duration and amplitude of the associated 5-HT transients
were increased by glucose stimulation. The amount of 5-HT released per secretion cycle was linearly related to the duration
of the underlying [Ca 2+ ] i oscillations in both 11 and 20 mM glucose. The slopes of the straight lines were identical, indicating that there is no significant
difference between the ability of calcium oscillations to elicit 5-HT/insulin release in 11 and 20 mM glucose.
In situ 5-HT microamperometry has the potential to resolve the high-frequency oscillatory component of the second phase of glucose-induced
insulin secretion. This component appears to reflect primarily the duration of the underlying [Ca 2+ ] i oscillations, suggesting that glucose metabolism and/or access to glucose metabolites is not rate limiting to fast pulsatile
insulin release. |
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ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1111/j.1469-7793.1998.135bz.x |