Sensing Escherichia coli O157：H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance （QCM） biosensor for Escherichia coli O157：H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid （MHDA） was introduced onto the Au surface of QCM, an...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2008-02, Vol.9 (2), p.121-131
Main Authors: Wang, Li-jiang, Wu, Chun-sheng, Hu, Zhao-ying, Zhang, Yuan-fan, Li, Rong, Wang, Ping
Format: Article
Language:English
Subjects:
Analytical chemistry
Antibodies
Antibodies - chemistry
Bacteria
Biomedical and Life Sciences
Biomedicine
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biotechnology
Colony Count, Microbial
Crystallization
E coli
Electrochemistry
Electrolytes
Equipment Design
Escherichia coli
Escherichia coli O157 - metabolism
Gold - chemistry
Immunoassay - instrumentation
Immunoassay - methods
LBL-SA
Oxidation-Reduction
QCM
Quartz
Signal Processing, Computer-Assisted
Software
Time Factors
大肠杆菌
生理传感器
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Summary:By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance （QCM） biosensor for Escherichia coli O157：H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid （MHDA） was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide （NHS） raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor＇s resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×10^2 colony forming units （CFU）/ml E. coli O157：H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly （LBL-SA） method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B0710307