Characterization of marginal zone B cell precursors

Selection of recently formed B cells into the follicular or marginal zone (MZ) compartments is proposed to occur by way of proliferative intermediates expressing high levels of CD21/35 and CD23. However, we show that CD21/35(high) CD23(+) splenocytes are not enriched for proliferative cells, and do...

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Bibliographic Details
Published in:	The Journal of experimental medicine 2005-11, Vol.202 (9), p.1225-1234
Main Authors:	Srivastava, Bhaskar, Quinn, 3rd, William J, Hazard, Kristin, Erikson, Jan, Allman, David
Format:	Article
Language:	eng
Subjects:	Animals B-Lymphocytes - cytology B-Lymphocytes - immunology B-Lymphocytes - metabolism Bromodeoxyuridine - metabolism Cell Differentiation - immunology Cell Proliferation Cells, Cultured Kinetics Membrane Glycoproteins - metabolism Mice Mice, Inbred C57BL Mice, Knockout Receptors, Complement - metabolism Receptors, Complement 3b - metabolism Receptors, Complement 3d - metabolism Receptors, IgE - metabolism Spleen - cytology Spleen - immunology Spleen - metabolism Stem Cells - metabolism Stem Cells - physiology
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Summary:	Selection of recently formed B cells into the follicular or marginal zone (MZ) compartments is proposed to occur by way of proliferative intermediates expressing high levels of CD21/35 and CD23. However, we show that CD21/35(high) CD23(+) splenocytes are not enriched for proliferative cells, and do not contribute substantially to the generation of follicular B cells. Instead, ontogenic relationships, steady-state labeling kinetics, and adoptive transfer experiments suggest that CD21/35(high) CD23(+) splenocytes serve primarily as precursors for MZ B cells, although their developmental potential seems to be broader and is influenced by environmental cues that are associated with lymphopenia. Furthermore, CD21/35(high) CD23(+) splenocytes share several key functional characteristics with MZ B cells, including their capacity to trap T-independent antigen and a heightened proliferative response to LPS. These observations challenge previous models of peripheral B cell maturation, and suggest that MZ B cells develop by way of CD21/35(high) CD23(+) intermediates.
ISSN:	0022-1007 1540-9538