C-C chemokines released by lipopolysaccharide (LPS)-stimulated human macrophages suppress HIV-1 infection in both macrophages and T cells

Human immunodeficiency virus-1 (HIV-1) expression in monocyte-derived macrophages (MDM) infected in vitro is known to be inhibited by lipopolysaccharide (LPS). However, the mechanisms are incompletely understood. We show here that HIV-1 suppression is mediated by soluble factors released by MDM stim...

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Bibliographic Details
Published in:The Journal of experimental medicine 1997-03, Vol.185 (5), p.805-816
Main Authors: Verani, A, Scarlatti, G, Comar, M, Tresoldi, E, Polo, S, Giacca, M, Lusso, P, Siccardi, A G, Vercelli, D
Format: Article
Language:English
Subjects:
AIDS/HIV
Cells, Cultured
Chemokine CCL4
Chemokine CCL5 - metabolism
Chemokine CCL5 - pharmacology
Chemokines - metabolism
Chemokines - pharmacology
DNA, Viral - analysis
Enzyme-Linked Immunosorbent Assay
HIV-1 - drug effects
HIV-1 - growth & development
Humans
Interleukin-6 - pharmacology
Lipopolysaccharide Receptors - biosynthesis
Lipopolysaccharides - pharmacology
Macrophage Inflammatory Proteins - metabolism
Macrophage Inflammatory Proteins - pharmacology
Macrophages - drug effects
Macrophages - virology
Polymerase Chain Reaction
Receptors, CCR5
Receptors, Cytokine - biosynthesis
Receptors, HIV - biosynthesis
T-Lymphocytes - drug effects
T-Lymphocytes - virology
Tumor Necrosis Factor-alpha - pharmacology
Up-Regulation
Virus Replication - drug effects
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Summary:Human immunodeficiency virus-1 (HIV-1) expression in monocyte-derived macrophages (MDM) infected in vitro is known to be inhibited by lipopolysaccharide (LPS). However, the mechanisms are incompletely understood. We show here that HIV-1 suppression is mediated by soluble factors released by MDM stimulated with physiologically significant concentrations of LPS. LPS-conditioned supernatants from MDM inhibited HIV-1 replication in both MDM and T cells. Depletion of C-C chemokines (RANTES, MIP-1 alpha, and MIP-1 beta) neutralized the ability of LPS-conditioned supernatants to inhibit HIV-1 replication in MDM. A combination of recombinant C-C chemokines blocked HIV-1 infection as effectively as LPS. Here, we report an inhibitory effect of C-C chemokines on HIV replication in primary macrophages. Our results raise the possibility that monocytes may play a dual role in HIV infection: while representing a reservoir for the virus, they may contribute to the containment of the infection by releasing factors that suppress HIV replication not only in monocytes but also in T lymphocytes.
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.185.5.805