Inhibition of murine B and T lymphopoiesis in vivo by an anti-interleukin 7 monoclonal antibody

The effects of interleukin 7 (IL-7) on the growth and differentiation of murine B cell progenitors has been well characterized using in vitro culture methods. We have investigated the role of IL-7 in vivo using a monoclonal antibody that neutralizes IL-7. We find that treatment of mice with this ant...

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Bibliographic Details
Published in:The Journal of experimental medicine 1993-07, Vol.178 (1), p.257-264
Main Authors: GRABSTEIN, K. H, WALDSCHMIDT, T. J, FINKELMAN, F. D, HESS, B. W, ALPERT, A. R, BOIANI, N. E, NAMEN, A. E, MORRISSEY, P. J
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibodies, Anti-Idiotypic - immunology
Antibodies, Monoclonal - immunology
B-Lymphocytes - physiology
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hematopoiesis
Immunobiology
Immunoglobulin M - analysis
Interleukin-7 - immunology
Interleukin-7 - physiology
Leukocyte Common Antigens - analysis
Lymphokines, interleukins ( function, expression)
Mice
Mice, Inbred BALB C
Regulatory factors and their cellular receptors
T-Lymphocytes - physiology
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Summary:The effects of interleukin 7 (IL-7) on the growth and differentiation of murine B cell progenitors has been well characterized using in vitro culture methods. We have investigated the role of IL-7 in vivo using a monoclonal antibody that neutralizes IL-7. We find that treatment of mice with this antibody completely inhibits the development of B cell progenitors from the pro-B cell stage forward. We also provide evidence that all peripheral B cells, including those of the B-1 and conventional lineages, are derived from IL-7-dependent precursors. The results are consistent with the rapid turnover of B cell progenitors in the marrow, but a slow turnover of mature B cells in the periphery. In addition to effects on B cell development, anti-IL-7 treatment substantially reduced thymus cellularity, affecting all major thymic subpopulations.
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.178.1.257