Autoantibodies specific for different isoforms of CD45 in systemic lupus erythematosus

Nearly one-third of IgM antilymphocyte autoantibody-positive sera from patients with systemic lupus erythematosus (SLE) contain IgM antibodies to one or more 180-220-kD molecules (p180, p190, p205, and p220) in blots of glycoproteins purified from T cells by wheat germ agglutinin affinity chromatogr...

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Bibliographic Details
Published in:The Journal of experimental medicine 1990-08, Vol.172 (2), p.653-656
Main Authors: Mimura, T, Fernsten, P, Jarjour, W, Winfield, J B
Format: Article
Language:English
Subjects:
Antigens, CD - immunology
Antigens, Differentiation - immunology
autoantibodies
Autoantibodies - analysis
autoimmune diseases
CD45 antigen
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Fluorescent Antibody Technique
Histocompatibility Antigens - immunology
Humans
Immunoblotting
Immunoglobulin M - analysis
Leukocyte Common Antigens
Lupus Erythematosus, Systemic - immunology
lymphocytes T
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Summary:Nearly one-third of IgM antilymphocyte autoantibody-positive sera from patients with systemic lupus erythematosus (SLE) contain IgM antibodies to one or more 180-220-kD molecules (p180, p190, p205, and p220) in blots of glycoproteins purified from T cells by wheat germ agglutinin affinity chromatography. Identity of these IgM targets with multiple isoforms of CD45 was established by their specific depletion from T cell glycoproteins by immunoprecipitation with T191, a monoclonal antibody (mAb) that reacts with an epitope common to all CD45 isoforms. Although the anti-CD45 autoantibodies recognize higher molecular weight isoforms primarily, antigenic specificity in this system is quite heterogeneous and includes multiple distinct CD45 isoforms on different types of T cells that are, at least in part, different from those reactive with mAbs 2H4 and UCHL-1. Because CD45 is a major membrane protein tyrosine phosphatase that plays a critical role in antigen-induced T cell activation, the present data may be relevant to some of the antilymphocyte antibody-mediated immunologic abnormalities that characterize SLE and related autoimmune diseases.
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.172.2.653