Identification of a Novel Population of Muscle Stem Cells in Mice: Potential for Muscle Regeneration

Three populations of myogenic cells were isolated from normal mouse skeletal muscle based on their adhesion characteristics and proliferation behaviors. Although two of these populations displayed satellite cell characteristics, a third population of long-time proliferating cells expressing hematopo...

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Bibliographic Details
Published in:The Journal of cell biology 2002-05, Vol.157 (5), p.851-864
Main Authors: Qu-Petersen, Zhuqing, Deasy, Bridget, Jankowski, Ron, Ikezawa, Makato, Cummins, James, Pruchnic, Ryan, Mytinger, John, Cao, Baohong, Gates, Charley, Wernig, Anton, Huard, Johnny
Format: Article
Language:English
Subjects:
Animals
Biomarkers
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cell Division - drug effects
Cell Division - physiology
Cell Separation
Cell transplantation
Cells
Cellular biology
Cultured cells
Dystrophin - physiology
Endothelial Growth Factors - pharmacology
Hematopoietic Stem Cell Transplantation
Hematopoietic stem cells
Identification
In Vitro Techniques
Lymphokines - pharmacology
Mice
Mice, Inbred C57BL
Mice, Inbred mdx
Multipotent stem cells
Muscle Fibers, Skeletal - cytology
Muscle, Skeletal - cytology
Muscle, Skeletal - immunology
Muscle, Skeletal - physiology
Muscles
Muscular Dystrophy, Animal - pathology
Muscular system
Nerve Growth Factor - pharmacology
Regeneration - physiology
Rodents
Skeletal muscle
Skeletal muscle satellite cells
Stem Cell Transplantation
Stem cells
Stem Cells - cytology
Stem Cells - immunology
Transplantation
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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Summary:Three populations of myogenic cells were isolated from normal mouse skeletal muscle based on their adhesion characteristics and proliferation behaviors. Although two of these populations displayed satellite cell characteristics, a third population of long-time proliferating cells expressing hematopoietic stem cell markers was also identified. This third population comprises cells that retain their phenotype for more than 30 passages with normal karyotype and can differentiate into muscle, neural, and endothelial lineages both in vitro and in vivo. In contrast to the other two populations of myogenic cells, the transplantation of the long-time proliferating cells improved the efficiency of muscle regeneration and dystrophin delivery to dystrophic muscle. The long-time proliferating cells' ability to proliferate in vivo for an extended period of time, combined with their strong capacity for self-renewal, their multipotent differentiation, and their immune-privileged behavior, reveals, at least in part, the basis for the improvement of cell transplantation. Our results suggest that this novel population of muscle-derived stem cells will significantly improve muscle cell-mediated therapies.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200108150