Visual Snapshots of Intracellular Kinase Activity at the Onset of Mitosis

Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior...

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Bibliographic Details
Published in:Chemistry & biology 2007-11, Vol.14 (11), p.1254-1260
Main Authors: Dai, Zhaohua, Dulyaninova, Natalya G., Kumar, Sanjai, Bresnick, Anne R., Lawrence, David S.
Format: Article
Language:English
Subjects:
Cell Line
CHEMBIO
Mitosis
Protein Kinase Inhibitors - pharmacology
Protein Kinases - metabolism
SIGNALING
Spectrometry, Fluorescence
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Summary:Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC β isoform with subsequent NEB.
ISSN:1074-5521
1879-1301
DOI:10.1016/j.chembiol.2007.10.007