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Reduced expression of deleted colorectal carcinoma (DCC) protein in established colon cancers
Using a bacterial fusion protein, a deleted colorectal carcinoma (DCC)-specific monoclonal antibody (MAb) 127-22 was established. Although MAb 127-22 reacted with almost all normal tissues, it did not react or only weakly reacted with many cancer cell lines, including colonic cancer lines, in flow c...
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Published in: | British journal of cancer 1998-02, Vol.77 (3), p.466-471 |
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container_title | British journal of cancer |
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creator | GOI, T YAMAGUCHI, A NAKAGAWARA, G URANO, T SHIKU, H FURUKAWA, K |
description | Using a bacterial fusion protein, a deleted colorectal carcinoma (DCC)-specific monoclonal antibody (MAb) 127-22 was established. Although MAb 127-22 reacted with almost all normal tissues, it did not react or only weakly reacted with many cancer cell lines, including colonic cancer lines, in flow cytometry. In Western immunoblots, the MAb reacted with a single 190-kDa molecule in a myeloma line Ara-10 extract. This component was scarcely detected in colonic cancer cell lines. Immunoblots of samples from 25 pairs of colonic cancers and adjacent normal tissues and from five adenoma tissues revealed that all normal colonic and adenoma tissues significantly expressed the DCC protein, whereas colonic cancer tissues showed poor expression. These results indicate not only deletion of and lowered mRNA expression of the DCC gene, but also marked reduction of DCC protein occurred in colonic cancer tissues. In addition, colonic cancer patients with liver metastasis expressed significantly lower levels of DCC than those without, suggesting the prognostic value of DCC expression. |
doi_str_mv | 10.1038/bjc.1998.74 |
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Although MAb 127-22 reacted with almost all normal tissues, it did not react or only weakly reacted with many cancer cell lines, including colonic cancer lines, in flow cytometry. In Western immunoblots, the MAb reacted with a single 190-kDa molecule in a myeloma line Ara-10 extract. This component was scarcely detected in colonic cancer cell lines. Immunoblots of samples from 25 pairs of colonic cancers and adjacent normal tissues and from five adenoma tissues revealed that all normal colonic and adenoma tissues significantly expressed the DCC protein, whereas colonic cancer tissues showed poor expression. These results indicate not only deletion of and lowered mRNA expression of the DCC gene, but also marked reduction of DCC protein occurred in colonic cancer tissues. 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Although MAb 127-22 reacted with almost all normal tissues, it did not react or only weakly reacted with many cancer cell lines, including colonic cancer lines, in flow cytometry. In Western immunoblots, the MAb reacted with a single 190-kDa molecule in a myeloma line Ara-10 extract. This component was scarcely detected in colonic cancer cell lines. Immunoblots of samples from 25 pairs of colonic cancers and adjacent normal tissues and from five adenoma tissues revealed that all normal colonic and adenoma tissues significantly expressed the DCC protein, whereas colonic cancer tissues showed poor expression. These results indicate not only deletion of and lowered mRNA expression of the DCC gene, but also marked reduction of DCC protein occurred in colonic cancer tissues. In addition, colonic cancer patients with liver metastasis expressed significantly lower levels of DCC than those without, suggesting the prognostic value of DCC expression.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Adhesion Molecules - immunology</subject><subject>Colonic Neoplasms - metabolism</subject><subject>DCC Receptor</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - secondary</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Receptors, Cell Surface</subject><subject>RNA, Messenger - analysis</subject><subject>Stomach. Duodenum. 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Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - secondary</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Receptors, Cell Surface</topic><topic>RNA, Messenger - analysis</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. 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subjects | Animals Antibodies, Monoclonal - immunology Biological and medical sciences Blotting, Western Cell Adhesion Molecules - analysis Cell Adhesion Molecules - genetics Cell Adhesion Molecules - immunology Colonic Neoplasms - metabolism DCC Receptor Gastroenterology. Liver. Pancreas. Abdomen Humans Immunohistochemistry Liver Neoplasms - metabolism Liver Neoplasms - secondary Medical sciences Mice Receptors, Cell Surface RNA, Messenger - analysis Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumor Suppressor Proteins Tumors |
title | Reduced expression of deleted colorectal carcinoma (DCC) protein in established colon cancers |
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