Reduced expression of deleted colorectal carcinoma (DCC) protein in established colon cancers

Using a bacterial fusion protein, a deleted colorectal carcinoma (DCC)-specific monoclonal antibody (MAb) 127-22 was established. Although MAb 127-22 reacted with almost all normal tissues, it did not react or only weakly reacted with many cancer cell lines, including colonic cancer lines, in flow c...

Full description

Saved in:
Bibliographic Details
Published in:British journal of cancer 1998-02, Vol.77 (3), p.466-471
Main Authors: GOI, T, YAMAGUCHI, A, NAKAGAWARA, G, URANO, T, SHIKU, H, FURUKAWA, K
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Biological and medical sciences
Blotting, Western
Cell Adhesion Molecules - analysis
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - immunology
Colonic Neoplasms - metabolism
DCC Receptor
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunohistochemistry
Liver Neoplasms - metabolism
Liver Neoplasms - secondary
Medical sciences
Mice
Receptors, Cell Surface
RNA, Messenger - analysis
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumor Suppressor Proteins
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using a bacterial fusion protein, a deleted colorectal carcinoma (DCC)-specific monoclonal antibody (MAb) 127-22 was established. Although MAb 127-22 reacted with almost all normal tissues, it did not react or only weakly reacted with many cancer cell lines, including colonic cancer lines, in flow cytometry. In Western immunoblots, the MAb reacted with a single 190-kDa molecule in a myeloma line Ara-10 extract. This component was scarcely detected in colonic cancer cell lines. Immunoblots of samples from 25 pairs of colonic cancers and adjacent normal tissues and from five adenoma tissues revealed that all normal colonic and adenoma tissues significantly expressed the DCC protein, whereas colonic cancer tissues showed poor expression. These results indicate not only deletion of and lowered mRNA expression of the DCC gene, but also marked reduction of DCC protein occurred in colonic cancer tissues. In addition, colonic cancer patients with liver metastasis expressed significantly lower levels of DCC than those without, suggesting the prognostic value of DCC expression.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1998.74