Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function

The septins are a family of proteins required for cytokinesis in a number of eukaryotic cell types. In budding yeast, these proteins are thought to be the structural components of a filament system present at the mother-bud neck, called the neck filaments. In this study, we report the isolation of a...

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Bibliographic Details
Published in:The Journal of cell biology 1998-11, Vol.143 (3), p.737-749
Main Authors: Frazier, J.A. (University of California at San Francisco, San Francisco, CA.), Wong, M.L, Longtine, M.S, Pringle, J.R, Mann, M, Mitchison, T.J, Field, C
Format: Article
Language:English
Subjects:
Antibodies
B lymphocytes
BINDING PROTEINS
BIOCHEMISTRY
BIOCHIMIE
BIOQUIMICA
CELL BUDDING
Cell Cycle Proteins - genetics
Cell Cycle Proteins - isolation & purification
Cell Cycle Proteins - metabolism
CELL DIVISION
CELL NECK FILAMENTS
CELL STRUCTURE
Cell walls
Cells
Cellular biology
CYTOCHEMISTRY
Cytokinesis
Cytoskeletal Proteins
DELETIONS
DIVISION CELLULAIRE
DIVISION CELULAR
Elution
ESTRUCTURA CELULAR
Fungal Proteins - genetics
Fungal Proteins - isolation & purification
Fungal Proteins - metabolism
Gels
GTP Phosphohydrolases
GTP-Binding Proteins - analysis
IMMUNOCYTOCHEMISTRY
IMMUNOLOGIE
IMMUNOLOGY
INMUNOLOGIA
INTERACTIONS
Membrane Proteins
MUTANT
MUTANTES
MUTANTS
Plasmids
POLIMERIZACION
POLYMERISATION
POLYMERIZATION
Polymers
Profilins
PROTEINAS AGLUTINANTES
PROTEINE DE LIAISON
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - isolation & purification
Recombinant Fusion Proteins - metabolism
Regular
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Schizosaccharomyces pombe Proteins
SEPTIN FILAMENTS
STRUCTURE CELLULAIRE
Transcription Factors
Yeast
Yeasts
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Description
Summary:The septins are a family of proteins required for cytokinesis in a number of eukaryotic cell types. In budding yeast, these proteins are thought to be the structural components of a filament system present at the mother-bud neck, called the neck filaments. In this study, we report the isolation of a protein complex containing the yeast septins Cdc3p, Cdc10p, Cdc11p, and Cdc12p that is capable of forming long filaments in vitro. To investigate the relationship between these filaments and the neck filaments, we purified septin complexes from cells deleted for CDC10 or CDC11. These complexes were not capable of the polymerization exhibited by wild-type preparations, and analysis of the neck region by electron microscopy revealed that the cdc10Δ and cdc11Δ cells did not contain detectable neck filaments. These results strengthen the hypothesis that the septins are the major structural components of the neck filaments. Surprisingly, we found that septin dependent processes like cytokinesis and the localization of Bud4p to the neck still occurred in cdc10Δ cells. This suggests that the septins may be able to function in the absence of normal polymerization and the formation of a higher order filament structure.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.143.3.737