Bim mediates mitochondria-regulated particulate matter-induced apoptosis in alveolar epithelial cells

We studied the role of Bim, a pro-apoptotic BCL-2 family member in Airborne particulate matter (PM 2.5μm)-induced apoptosis in alveolar epithelial cells (AEC). PM induced AEC apoptosis by causing significant reduction of mitochondrial membrane potential and increase in caspase-9, caspase-3 and PARP-...

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Bibliographic Details
Published in:FEBS letters 2007-09, Vol.581 (22), p.4148-4152
Main Authors: Zhang, J., Ghio, A.J., Chang, W., Kamdar, O., Rosen, G.D., Upadhyay, D.
Format: Article
Language:English
Subjects:
AEC
alveolar epithelial cells
Ambient air pollution particle
Animals
Apoptosis
Apoptosis - drug effects
Apoptosis Regulatory Proteins - genetics
Apoptosis Regulatory Proteins - metabolism
Bcl-2-Like Protein 11
Bim
Cattle
Cell Line, Tumor
DNA Fragmentation - drug effects
Epithelial Cells - cytology
Epithelial Cells - drug effects
Humans
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mitochondria - metabolism
Models, Biological
Particulate matter
Particulate Matter - pharmacology
Poly(ADP-ribose) Polymerases - genetics
pro-apoptotic BCL-2 family member
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Pulmonary Alveoli - cytology
Pulmonary Alveoli - drug effects
Rats
reactive oxygen species
RNA, Small Interfering - metabolism
ROS
Up-Regulation - drug effects
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Summary:We studied the role of Bim, a pro-apoptotic BCL-2 family member in Airborne particulate matter (PM 2.5μm)-induced apoptosis in alveolar epithelial cells (AEC). PM induced AEC apoptosis by causing significant reduction of mitochondrial membrane potential and increase in caspase-9, caspase-3 and PARP-1 activation. PM upregulated pro-apoptotic protein Bim and enhanced translocation of Bim to the mitochondria. ShRNABim blocked PM-induced apoptosis by preventing activation of the mitochondrial death pathway suggesting a role of Bim in the regulation of mitochondrial pathway in AEC. Accordingly, we provide the evidence that Bim mediates PM-induced apoptosis via mitochondrial pathway.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2007.07.080