A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium

Analysis of a developmental mutant in Dictyostelium discoideum which is unable to initiate morphogenesis has shown that a protein kinase of the MAP kinase/ERK family affects relay of the cAMP chemotactic signal and cell differentiation. Strains in which the locus encoding ERK2 is disrupted respond t...

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Bibliographic Details
Published in:The Journal of cell biology 1995-02, Vol.128 (3), p.405-413
Main Authors: Segall, J.E. (Albert Einstein College of Medicine, Bronx, NY.), Kuspa, A, Shaulsky, G, Ecke, M, Maeda, M, Gaskins, C, Firtel, R.A, Loomis, W.F
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ADENOSINE MONOPHOSPHATE
ADENOSINMONOFOSFATO
Adenylyl Cyclases - metabolism
ADN
Aggregation
Amino Acid Sequence
Animals
Cell aggregates
Cells
Cellular biology
Cellular differentiation
CHIMIORECEPTEUR
Complementary DNA
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DICTYOSTELIUM
Dictyostelium - enzymology
Dictyostelium discoideum
Enzyme Activation
Enzymes
FOSFORILACION
GENE
Gene expression regulation
GENES
Genetic vectors
GTP-Binding Proteins - metabolism
Mitogen-Activated Protein Kinase 1
Molecular Sequence Data
Mutation
NUCLEOTIDE
NUCLEOTIDOS
Phenotype
PHOSPHORYLATION
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Protein-Tyrosine Kinases - genetics
Protein-Tyrosine Kinases - metabolism
PROTEINA QUINASA
PROTEINE KINASE
Proteins
QUIMIORECEPTORES
Receptors
Receptors, Cytoplasmic and Nuclear - metabolism
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
TRANSFERASAS
TRANSFERASE
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Description
Summary:Analysis of a developmental mutant in Dictyostelium discoideum which is unable to initiate morphogenesis has shown that a protein kinase of the MAP kinase/ERK family affects relay of the cAMP chemotactic signal and cell differentiation. Strains in which the locus encoding ERK2 is disrupted respond to a pulse of cAMP by synthesizing cGMP normally but show little synthesis of cAMP. Since mutant cells lacking ERK2 contain normal levels of both the cytosolic regulator of adenylyl cyclase (CRAC) and manganese-activatable adenylyl cyclase, it appears that this kinase is important for receptor-mediated activation of adenylyl cyclase
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.128.3.405