The Expression of Slow Myosin during Mammalian Somitogenesis and Limb Bud Differentiation

The developmental pattern of slow myosin expression has been studied in mouse embryos from the somitic stage to the period of secondary fiber formation and in myogenic cells, cultured from the same developmental stages. The results obtained, using a combination of different polyclonal and monoclonal...

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Bibliographic Details
Published in:The Journal of cell biology 1988-12, Vol.107 (6), p.2191-2197
Main Authors: Vivarelli, E., Brown, W. E., Whalen, R. G., Cossu, G.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal - immunology
Biological and medical sciences
Blotting, Western
Cell culture techniques
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell lines
Cell physiology
Cells
Cells, Cultured
Cultured cells
Embryos
Extremities - embryology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Gestational Age
limbs
mammalian cells
Mice
Molecular and cellular biology
monoclonal antibodies
Muscle development
Muscle fibers
Muscles - embryology
Myoblasts
myosin
Myosins - physiology
Neurons
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Summary:The developmental pattern of slow myosin expression has been studied in mouse embryos from the somitic stage to the period of secondary fiber formation and in myogenic cells, cultured from the same developmental stages. The results obtained, using a combination of different polyclonal and monoclonal antibodies, indicate that slow myosin is coexpressed in virtually all the cells that express embryonic (fast) myosin in somites and limb buds in vivo as well as in culture. On the contrary fetal or late myoblasts (from 15-d-old embryos) express in culture only embryonic (fast) myosin. At this stage, muscle cells in vivo, as already shown, consist of primary myotubes, which express both myosins, and secondary myotubes, which express preferentially embryonic (fast) myosin. Under no circumstance neonatal or adult fast myosins were detected. Western blot analysis confirmed the immunocytochemical data. These results suggest that embryonic myoblasts in mammals are all committed to the mixed embryonic-(fast) slow lineage and, accordingly, all primary fibers express both myosins, whereas fetal myoblasts mostly belong to the embryonic (fast) lineage and likely generate fibers containing only embryonic (fast) myosin. The relationship with current models of avian myogenesis are discussed.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.107.6.2191