Characterization of the lipopolysaccharide O antigens of Actinobacillus pleuropneumoniae serotypes 9 and 11: antigenic relationships among serotypes 9, 11, and 1

The antigenic lipopolysaccharide O polysaccharides of capsular serotypes 9 and 11 were examined by chemical, immunological, and nuclear magnetic resonance methods. Immunodiffusion tests carried out on these O antigens indicated that both contained common epitopes which were also shared by Actinobaci...

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Bibliographic Details
Published in:Journal of Bacteriology 1992-08, Vol.174 (16), p.5324-5331
Main Authors: Beynon, L.M. (National Research Council of Canada, Ottawa, Ontario, Canada), Griffith, D.W, Richards, J.C, Perry, M.B
Format: Article
Language:English
Subjects:
ACTINOBACILLUS PLEUROPNEUMONIAE
Actinobacillus pleuropneumoniae - classification
Actinobacillus pleuropneumoniae - immunology
Bacteriology
Biological and medical sciences
Carbohydrate Sequence
CERDO
Electrophoresis, Polyacrylamide Gel
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Immunodiffusion
LIPOPOLISACARIDOS
LIPOPOLYSACCHARIDE
Magnetic Resonance Spectroscopy
Methylation
Microbiology
Molecular Sequence Data
Morphology, structure, chemical composition
O Antigens
Polysaccharides, Bacterial - analysis
Polysaccharides, Bacterial - immunology
PORCIN
SEROTIPOS
SEROTYPE
Serotyping
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Summary:The antigenic lipopolysaccharide O polysaccharides of capsular serotypes 9 and 11 were examined by chemical, immunological, and nuclear magnetic resonance methods. Immunodiffusion tests carried out on these O antigens indicated that both contained common epitopes which were also shared by Actinobacillus pleuropneumoniae serotype 1. Chemical analysis and high-field nuclear magnetic resonance spectroscopy showed that the O antigens of serotypes 9 and 11 were high-molecular-weight polymers consisting of a backbone of repeating trisaccharide units composed of alpha-L-rhamnopyranosyl and alpha-D-glucopyranosyl residues (2:1). One of the alpha-L-rhamnose units forms a branch point and is stoichiometrically substituted with terminal 2-acetamido-2-deoxy-beta-D-glucose residues in the serotype 11 O polysaccharide, but only to the extent of 25% in the serotype 9 O polysaccharide. Thus, the serotype 9 O polysaccharide contains two different repeating units: a tetrasaccharide unit with the same structure as that of the serotype 11 O polysaccharide and a trisaccharide unit: (...) where R = beta-D-GlcpNAc for serotype 1 and 11 O polysaccharides, and R = H (75%) and R = beta-D-GlcpNAc (25%) for serotype 9. The structure of the previously determined serotype 1 O polysaccharide (E. Altman, J.-R. Brisson, and M.B. Perry, Biochem. Cell Biol. 64:17-25, 1986) is identical to that of the serotype 11 O polysaccharide. We propose a more complete serotyping scheme for A. pleuropneumoniae which includes designation of both the capsular (K) and O antigens
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.174.16.5324-5331.1992