Midkine induces the transformation of NIH3T3 cells

Midkine (MK) is a heparin-binding growth factor and is frequently expressed at high levels in many human carcinomas. To investigate further the roles of MK in the regulation of cell growth, we introduced MK expression in NIH3T3 cells. A mixture of transfectants of an MK expression vector, but not a...

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Bibliographic Details
Published in:British journal of cancer 1997-01, Vol.75 (3), p.354-359
Main Authors: KADOMATSU, K, HAGIHARA, M, AKHTER, S, FAN, Q.-W, MURAMATSU, H, MURAMATSU, T
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Biological and medical sciences
Carrier Proteins - biosynthesis
Cell Division
Cell physiology
Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes
Cell Transformation, Neoplastic
Cytokines - biosynthesis
Drosophila Proteins
Fundamental and applied biological sciences. Psychology
Humans
Kinetics
Mice
Mice, Nude
Midkine
Molecular and cellular biology
Neoplasms, Experimental - pathology
Oncogenes
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins c-ret
Receptor Protein-Tyrosine Kinases - biosynthesis
Transfection
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Summary:Midkine (MK) is a heparin-binding growth factor and is frequently expressed at high levels in many human carcinomas. To investigate further the roles of MK in the regulation of cell growth, we introduced MK expression in NIH3T3 cells. A mixture of transfectants of an MK expression vector, but not a control vector, formed colonies in soft agar, showed an elevated cell number at confluence, and formed tumours in nude mice. An interesting characteristic of the transformed cells was that they became spontaneously detached from the culture dish substratum. In the transformed cells, MK was not only secreted, but also localized, in the perinuclear region as spots. The present data indicate that MK has the potential to transform NIH3T3 cells and suggest that overexpression of the MK gene may promote unregulated cell growth in vivo.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1997.58