Drosophila Spd-2 Recruits PCM to the Sperm Centriole, but Is Dispensable for Centriole Duplication

In C. elegans, genome-wide screens have identified just five essential centriole-duplication factors: SPD-2, ZYG-1, SAS-5, SAS-6, and SAS-4 [1–8]. These proteins are widely believed to comprise a conserved core duplication module [3, 9–14]. In worm embryos, SPD-2 is the most upstream component of th...

Full description

Saved in:
Bibliographic Details
Published in:Current biology 2007-10, Vol.17 (20), p.1759-1764
Main Authors: Dix, Carly I., Raff, Jordan W.
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans - genetics
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - metabolism
CELLBIO
CELLCYCLE
Centrioles - metabolism
Drosophila
Drosophila - genetics
Drosophila - metabolism
Drosophila - ultrastructure
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Fertilization - physiology
Male
Meiosis - physiology
Mitosis - physiology
Spermatozoa - metabolism
Spermatozoa - ultrastructure
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In C. elegans, genome-wide screens have identified just five essential centriole-duplication factors: SPD-2, ZYG-1, SAS-5, SAS-6, and SAS-4 [1–8]. These proteins are widely believed to comprise a conserved core duplication module [3, 9–14]. In worm embryos, SPD-2 is the most upstream component of this module, and it is also essential for pericentriolar material (PCM) recruitment to the centrioles [1, 4, 15, 16]. Here, we show that Drosophila Spd-2 (DSpd-2) is a component of both the centrioles and the PCM and has a role in recruiting PCM to the centrioles. DSpd-2 appears not, however, to be essential for centriole duplication in somatic cells. Moreover, PCM recruitment in DSpd-2 mutant somatic cells is only partially compromised, and mitosis appears unperturbed. In contrast, DSpd-2 is essential for proper PCM recruitment to the fertilizing sperm centriole, and hence for microtubule nucleation and pronuclear fusion. DSpd-2 therefore appears to have a particularly important role in recruiting PCM to the sperm centriole. We speculate that the SPD-2 family of proteins might only be absolutely essential for the recruitment of centriole duplication factors and PCM to the centriole(s) that enter the egg with the fertilizing sperm.
ISSN:0960-9822
1879-0445
DOI:10.1016/j.cub.2007.08.065