Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae

The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature termination codons (PTCs). In yeast Saccharomyces cerevisiae, the activity of the NMD pathway depends on the recognition of the PTC by the translational machinery. Translation termination fact...

Full description

Saved in:
Bibliographic Details
Published in:BMC molecular biology 2007-08, Vol.8 (1), p.71-71
Main Authors: Chabelskaya, Svetlana, Gryzina, Valentina, Moskalenko, Svetlana, Le Goff, Catherine, Zhouravleva, Galina
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing
Antisense RNA
Base Sequence
Biochemistry, Molecular Biology
Brewer's yeast
Codon, Nonsense
Codon, Nonsense - genetics
Down-Regulation
Gene Deletion
Gene Expression Regulation, Fungal
Gene mutations
Genetic aspects
Health aspects
Life Sciences
Molecular Sequence Data
Peptide Termination Factors
Peptide Termination Factors - genetics
Peptide Termination Factors - metabolism
Phenotype
RNA Helicases
RNA Helicases - genetics
RNA Helicases - metabolism
RNA Stability
RNA Stability - genetics
RNA, Messenger
RNA, Messenger - genetics
RNA-Binding Proteins
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Trans-Activators
Trans-Activators - genetics
Trans-Activators - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature termination codons (PTCs). In yeast Saccharomyces cerevisiae, the activity of the NMD pathway depends on the recognition of the PTC by the translational machinery. Translation termination factors eRF1 (Sup45) and eRF3 (Sup35) participate not only in the last step of protein synthesis but also in mRNA degradation and translation initiation via interaction with such proteins as Pab1, Upf1, Upf2 and Upf3. In this work we have used previously isolated sup45 mutants of S. cerevisiae to characterize degradation of aberrant mRNA in conditions when translation termination is impaired. We have sequenced his7-1, lys9-A21 and trp1-289 alleles which are frequently used for analysis of nonsense suppression. We have established that sup45 nonsense and missense mutations lead to accumulation of his7-1 mRNA and CYH2 pre-mRNA. Remarkably, deletion of the UPF1 gene suppresses some sup45 phenotypes. In particular, sup45-n upf1Delta double mutants were less temperature sensitive, and more resistant to paromomycin than sup45 single mutants. In addition, deletion of either UPF2 or UPF3 restored viability of sup45-n double mutants. This is the first demonstration that sup45 mutations do not only change translation fidelity but also acts by causing a change in mRNA stability.
ISSN:1471-2199
1471-2199
DOI:10.1186/1471-2199-8-71