A biomimetic peptide fluorosurfactant polymer for endothelialization of ePTFE with limited platelet adhesion

Abstract Endothelialization of expanded polytetrafluoroethylene (ePTFE) has the potential to improve long-term patency for small-diameter vascular grafts. Successful endothelialization requires ePTFE surface modification to permit cell attachment to this otherwise non-adhesive substrate. We report h...

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Bibliographic Details
Published in:Biomaterials 2007-08, Vol.28 (24), p.3537-3548
Main Authors: Larsen, Coby C, Kligman, Faina, Tang, Chad, Kottke-Marchant, Kandice, Marchant, Roger E
Format: Article
Language:English
Subjects:
Advanced Basic Science
Amino Acid Sequence
Blood Platelets - cytology
Cell Adhesion
Cells, Cultured
Dentistry
Endothelial cells
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
ePTFE vascular grafts
Humans
Molecular Mimicry
Molecular Sequence Data
Platelet adhesion
Polymers - chemistry
Polytetrafluoroethylene - pharmacology
Spectrophotometry, Ultraviolet
Surface modification
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Summary:Abstract Endothelialization of expanded polytetrafluoroethylene (ePTFE) has the potential to improve long-term patency for small-diameter vascular grafts. Successful endothelialization requires ePTFE surface modification to permit cell attachment to this otherwise non-adhesive substrate. We report here on a peptide fluorosurfactant polymer (FSP) biomimetic construct that promotes endothelial cell (EC)-selective attachment, growth, shear stability, and function on ePTFE. The peptide FSP consists of a flexible poly(vinyl amine) backbone with EC-selective peptide ligands for specific cell adhesion and pendant fluorocarbon branches for stable anchorage to underlying ePTFE. The EC-selective peptide (primary sequence: Cys–Arg–Arg–Glu–Thr–Ala–Trp–Ala–Cys, CRRETAWAC) has demonstrated high binding affinity for the α5 β1 integrin found on ECs. Here, we demonstrate low affinity of CRRETAWAC for platelets and platelet integrins, thus providing it with EC-selectivity. This EC-selectivity could potentially facilitate rapid in vivo endothelialization and healing without thrombosis for small-diameter ePTFE vascular grafts.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2007.04.026